We narrowed to 10,449 results for: T7

1. hSRAP.TWIN1.a13-69

   Plasmid

   #49815

   Purpose

   expression vector for SRAP(13-69) in pTWIN1 vector

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator protein aa 13-69 (SRA1 Human)
   

   Use

   Tags

   intein & chitin binding domain

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p28BIOHTEV-LIC: nsp16_SARS-CoV-2

   Plasmid

   #167846

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp16

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp16
   

   Use

   Tags

   6xHis and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

   Plasmid

   #123341

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-EKAR-EV
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. His-MBP-NRBF2 (1-159)

   Plasmid

   #99331

   Purpose

   Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

   Depositor

   James Hurley

   Article

   Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

   Insert

   Nuclear receptor-binding factor 2 (NRBF2 Human)
   

   Use

   Tags

   6xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   AA1-159 are present

   Promoter

   T7

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pet28a_RBPMS_1_129

   Plasmid

   #65738

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 1-129

   Promoter

   T7

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETDuet-msGC-NT21

   Plasmid

   #78102

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 1-380 of the beta subunit and fragment 2…

   Promoter

   T7

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HOXB4 (human) HIS-tag pET new RBS

   Plasmid

   #8525

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Contains improved Ribosomal binding site; makes …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pet28a_RBPMS_1_100

   Plasmid

   #65735

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 1-100

   Promoter

   T7

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

   Plasmid

   #101208

   Purpose

   Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N497A, R661A and Q695A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pbIG-Xph20-ETWV

    Plasmid

    #133034

    Purpose

    Expresses Xph20-ETWV in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Use

    Tags

    AviTag, His10, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTAMAHISTEV_PfeAR480A-Q482A

    Plasmid

    #128946

    Purpose

    expresses R480A Q482A mutant of PfeA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

    Insert

    ferric enterobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-75), …

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-Drp1(deltaIB)

    Plasmid

    #73041

    Purpose

    Bacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Drp1(deltaIB) (Dnm1l Mouse)
    

    Use

    Tags

    6xHis-tag and PreScission Protease site

    Expression

    Bacterial

    Mutation

    Deleted amino acids 514-602

    Promoter

    T7

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Zebrafish-gRNA-0003

    Plasmid

    #42243

    Purpose

    gRNA targeted to zebrafish gene fh [site #1]

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-fh site #1 (fh Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

14. Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

    Plasmid

    #111052

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AB (325-557, Δ 368-523)

    Promoter

    T7

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET15b-ITPA_P32T

    Plasmid

    #60825

    Purpose

    to purify human ITPA from bacteria

    Depositor

    Youri Pavlov

    Article

    Stepchenkova et al J Mol Biol. 2009 Sep 25;392(3):602-13. d

    Insert

    ITPA (ITPA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P32T

    Promoter

    T7

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. M26I DJ-1 TEV site pET15b

    Plasmid

    #60685

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pETNKI-his-3C-LIC-Amp

    Plasmid

    #108704

    Purpose

    Expression of a His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28b-hTIG3-1-164

    Plasmid

    #100724

    Purpose

    Full-length human TIG3 in E.coli expression vector

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    RARRES3 (PLAAT4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pet23a_RBPMS_1_120

    Plasmid

    #65730

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 1-120

    Promoter

    T7

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX6P1-human LIC1 G domain

    Plasmid

    #74598

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28b-hTIG3-1-132

    Plasmid

    #100720

    Purpose

    Expression of N-terminal region of human TIG3 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    RARRES3 (PLAAT4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid

    #101229

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pet23a_RBPMS_20_101

    Plasmid

    #65733

    Purpose

    plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    deletion construct encoding amino acids 20-101

    Promoter

    T7

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET-22HT-MSI1(7-192)R53E/R59E/R61E

    Plasmid

    #60355

    Purpose

    R53E R59E R61E triple mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E, R59E and R61E mutations; contains amino aci…

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

27. hSRAP.TWIN1.a13-87

    Plasmid

    #49816

    Purpose

    expression plasmid for SRAP(13-87) in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 13-87 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMCSG49-AlleyCat7

    Plasmid

    #42591

    Depositor

    Ivan Korendovych

    Article

    Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

    Insert

    mutated C-terminal portion of calmodulin gene (M76-K148)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Isole…

    Promoter

    T7

    Available since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hSRAP.TWIN1.a13-215

    Plasmid

    #49817

    Purpose

    expression vector for SRAP(13-215) in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 13-215 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GIL-6

    Plasmid

    #213389

    Purpose

    Expresses a chimeric cytokine between IL-6 and LIF

    Depositor

    Jurgen Scheller

    Article

    Rafii et al J Biol Chem. 2024 May;300(5):107251. doi

    Insert

    GIL-6
    

    Use

    Tags

    IgG1-Fc-tag and Myc-tg

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GIO-6

    Plasmid

    #213390

    Purpose

    Expresses a chimeric cytokine between IL-6 and OSM

    Depositor

    Jurgen Scheller

    Article

    Rafii et al J Biol Chem. 2024 May;300(5):107251. doi

    Insert

    GIO-6
    

    Use

    Tags

    IgG1-Fc-tag and Myc-tg

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. FUS LC TtoS

    Plasmid

    #226608

    Purpose

    All threonines to serine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC 4QQ->GG (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    4 QQ pairs replaced with GG pairs

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FUS LC QtoA

    Plasmid

    #226609

    Purpose

    All glutamines to alanine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    6*His-TEV-FUS LC QtoA (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All glutamine to alanine

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FUS LC-RGG1 StoG

    Plasmid

    #226610

    Purpose

    All serines in the LC domain to glycine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC-RGG1 StoG (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All serines to glycine in the LC domain

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FUS LC-RGG1 TtoS

    Plasmid

    #226611

    Purpose

    All threonines in the LC domain to serine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC-RGG1 TtoS (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All threonine to serine in the LC domain

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FUS LC-RGG1 QtoA

    Plasmid

    #226612

    Purpose

    All glutamines in the LC domain to alanine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC-RGG1 QtoA (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All glutamine to alanine in the LC domain

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FUS LC-RGG1 TtoV

    Plasmid

    #226613

    Purpose

    All threonines in the LC domain to valine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC-RGG1 TtoV (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All threonine to valine in the LC domain

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FUS LC-RGG1 QtoG

    Plasmid

    #226614

    Purpose

    All glutamines in the LC domain to glycine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC-RGG1 QtoG (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All glutamine to glycine in the LC domain

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FUS LC-RGG1 QtoN

    Plasmid

    #226615

    Purpose

    All glutamines in the LC domain to asparagine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC-RGG1 QtoN (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All glutamine to asparagine in the LC domain

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FUS LC-RGG1 QtoS

    Plasmid

    #226616

    Purpose

    All glutamines in the LC domain to serine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    FUS LC-RGG1 QtoS (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All glutamine to serine in the LC domain

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge