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Showing: 11451 - 11500 of 23409 results

  1. pENTRY4_ PLDbeta1

    Plasmid
    #126902
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126902-plasmid-map-sequence-id-249806

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  2. pENTRY4_ OBfold

    Plasmid
    #126901
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126901-plasmid-map-sequence-id-249798

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  3. pICSL4723_ OBfold

    Plasmid
    #126889
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126889-plasmid-map-sequence-id-249747

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  4. pENTRY4_ HDT701

    Plasmid
    #126898
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126898-plasmid-map-sequence-id-249718

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  5. pENTRY4_ Dja6_1

    Plasmid
    #126894
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126894-plasmid-map-sequence-id-249699

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  6. pENTRY4_ ERF922

    Plasmid
    #126893
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126893-plasmid-map-sequence-id-249692

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  7. A6TSR

    Plasmid
    #50359
    Purpose
    expresses T.gondii MIC2(67-660) domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-A6TSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. plenti guide Thy1.1

    Plasmid
    #128063
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128063-plasmid-map-sequence-id-255154

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  9. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  10. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid
    #192664
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB-MeCP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192664-plasmid-map-sequence-id-379868

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  11. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid
    #160629
    Purpose
    TU for the expression of LbCas12a
    Depositor
    Diego Orzaez
    Insert
    AtUBQ10-:LbCpf1(Cas12a):Tnos
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    UBQ10
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160629-plasmid-map-sequence-id-321990

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  12. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  13. PX380 Treponema denticola Cas9

    Plasmid
    #68317
    Purpose
    Treponema denticola Cas9
    Depositor
    Feng Zhang
    Insert
    TdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68317-plasmid-map-sequence-id-119529

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  14. PX404 Campylobacter jejuni Cas9

    Plasmid
    #68338
    Purpose
    Campylobacter jejuni Cas9
    Depositor
    Feng Zhang
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    HA-NLS and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68338-plasmid-map-sequence-id-119561

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  15. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  16. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid
    #192667
    Purpose
    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192667-plasmid-map-sequence-id-379870

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  17. plenti guide ds red

    Plasmid
    #128055
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128055-plasmid-map-sequence-id-255092

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  18. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid
    #191388
    Purpose
    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_hSLBP(56_108)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191388-plasmid-map-sequence-id-378110

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  20. PX381 Filifactor alocis Cas9

    Plasmid
    #68318
    Purpose
    Filifactor alocis Cas9
    Depositor
    Feng Zhang
    Insert
    FaCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68318-plasmid-map-sequence-id-119531

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  22. PX385 Streptococcus pasteurianus Cas9

    Plasmid
    #68322
    Purpose
    Streptococcus pasteurianus Cas9
    Depositor
    Feng Zhang
    Insert
    SpaCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68322-plasmid-map-sequence-id-119537

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  23. PX396 Parvibaculum lavamentivorans Cas9

    Plasmid
    #68333
    Purpose
    Parvibaculum lavamentivorans Cas9
    Depositor
    Feng Zhang
    Insert
    PlCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68333-plasmid-map-sequence-id-119549

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  24. PX377 Corynebacter diphtheriae Cas9

    Plasmid
    #68314
    Purpose
    Corynebacter diphtheriae Cas9
    Depositor
    Feng Zhang
    Insert
    CdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68314-plasmid-map-sequence-id-119522

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  25. PX405 Neisseria meningitidis Cas9

    Plasmid
    #68702
    Purpose
    Neisseria meningitidis Cas9
    Depositor
    Feng Zhang
    Insert
    NmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. PX394 Neisseria cinerea Cas9

    Plasmid
    #68331
    Purpose
    Neisseria cinerea Cas9
    Depositor
    Feng Zhang
    Insert
    NcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68331-plasmid-map-sequence-id-119554

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  27. PX406 Pasteurella multocida Cas9

    Plasmid
    #68703
    Purpose
    Pasteurella multocida Cas9
    Depositor
    Feng Zhang
    Insert
    PmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. pSBtet-RB-PE2

    Plasmid
    #173903
    Purpose
    SB-transposon with inducible expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173903-plasmid-map-sequence-id-346343

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  29. PX390 Flavobacterium columnare Cas9

    Plasmid
    #68327
    Purpose
    Flavobacterium columnare Cas9
    Depositor
    Feng Zhang
    Insert
    FcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68327-plasmid-map-sequence-id-119543

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  30. pCDNA3.3 CAS9 IRES-Dsred

    Plasmid
    #129444
    Purpose
    expression of FnCas9 and DsRed
    Depositor
    Oskar Laur
    Insert
    FnCas9 IRES-Dsred
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129444-plasmid-map-sequence-id-256492

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  31. PX383 Lactobacillus johnsonii Cas9

    Plasmid
    #68320
    Purpose
    Lactobacillus johnsonii Cas9
    Depositor
    Feng Zhang
    Insert
    LjCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68320-plasmid-map-sequence-id-119535

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  32. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid
    #160587
    Purpose
    Nickase D10A Cas9 for Nt and Ct fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160587-plasmid-map-sequence-id-322079

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  34. PX386 Lactobacillus farciminis Cas9

    Plasmid
    #68323
    Purpose
    Lactobacillus farciminis Cas9
    Depositor
    Feng Zhang
    Insert
    LfCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68323-plasmid-map-sequence-id-119538

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  35. PX395 Roseburia intestinalis Cas9

    Plasmid
    #68332
    Purpose
    Roseburia intestinalis Cas9
    Depositor
    Feng Zhang
    Insert
    RiCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68332-plasmid-map-sequence-id-119548

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  37. PX389 Fluviicola taffensis Cas9

    Plasmid
    #68326
    Purpose
    Fluviicola taffensis Cas9
    Depositor
    Feng Zhang
    Insert
    FtCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68326-plasmid-map-sequence-id-119542

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  38. PX388 Bacteroides coprophilus Cas9

    Plasmid
    #68325
    Purpose
    Bacteroides coprophilus Cas9
    Depositor
    Feng Zhang
    Insert
    BcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68325-plasmid-map-sequence-id-125196

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  39. PX378 Sutterella wadsworthensis Cas9

    Plasmid
    #68315
    Purpose
    Sutterella wadsworthensis Cas9
    Depositor
    Feng Zhang
    Insert
    SwCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68315-plasmid-map-sequence-id-119526

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  40. PX387 Mycoplasma mobile Cas9

    Plasmid
    #68324
    Purpose
    Mycoplasma mobile Cas9
    Depositor
    Feng Zhang
    Insert
    MmCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    68324-plasmid-map-sequence-id-119539

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  41. PX392 Azospirillum B510 Cas9

    Plasmid
    #68329
    Purpose
    Azospirillum B510 Cas9
    Depositor
    Feng Zhang
    Insert
    AzoCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68329-plasmid-map-sequence-id-119551

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  42. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Use
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid
    #221385
    Purpose
    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene
    Depositor
    Junwei Shi
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    221385-plasmid-map-sequence-id-435944

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  44. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173902-plasmid-map-sequence-id-346342

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  45. pUPD2 nickase D10A Cas9 (GB1691)

    Plasmid
    #160588
    Purpose
    Nickase D10A Cas9 for Nt fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160588-plasmid-map-sequence-id-322078

    Enlarge

  46. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  47. pX335 Mouse 5' Srcap gRNA B

    Plasmid
    #127905
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127905-plasmid-map-sequence-id-249688

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  48. pX335 Mouse 5' Srcap gRNA A

    Plasmid
    #127904
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Mark Groudine
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127904-plasmid-map-sequence-id-249683

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  49. pX330 Mouse 3' Ubf gRNA

    Plasmid
    #127902
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene
    Depositor
    Mark Groudine
    Insert
    gRNA for Mouse 3' Ubf
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127902-plasmid-map-sequence-id-249774

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  50. pZDonor-U6-myogenin stop codon gRNA

    Plasmid
    #69549
    Purpose
    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Insert
    Myogenin C terminus-targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69549-plasmid-map-sequence-id-122972

    Enlarge

Showing: 11451 - 11500 of 23409 results