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We narrowed to 12,455 results for: cel.2;

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Showing: 11451 - 11500 of 12455 results

  2. LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

    Plasmid
    #170226
    Purpose
    Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    IKBKB (IKBKB Human)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    S177E, S181E
    Promoter
    EF1a
    Available Since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170226-plasmid-map-sequence-id-334494

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  4. pcDNA3.1-FLT3-D835N-V5/HIS

    Plasmid
    #236008
    Purpose
    expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835N substitution
    Promoter
    CMV
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236008-plasmid-map-sequence-id-469587

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  8. 3'-ecRESCUE-mCherry

    Plasmid
    #191486
    Purpose
    ecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherry
    Depositor
    Xu Ma
    Article
    Wang et al Cell Commun Signal. 2021 Jul 31;19(1):81
    Insert
    dRanCas13b-hADAR2-mCherry-ecDHFR (folA, ADARB1 Human, R. anatipestifer, E. coli)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191486-plasmid-map-sequence-id-374351

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  11. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

    Plasmid
    #217368
    Purpose
    Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV
    Depositor
    Abhishek Chatterjee
    Article
    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2
    Insert
    E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
    Use
    AAV
    Expression
    Mammalian
    Mutation
    C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C
    Promoter
    U6
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    217368-plasmid-map-sequence-id-430295

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  13. pcDNA3.1-PDGFRA-R487L-V5/HIS

    Plasmid
    #234761
    Purpose
    Expression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal Tumor
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    R487L substitution
    Promoter
    CMV
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234761-plasmid-map-sequence-id-468953

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  15. pcDNA3.1-PDGFRA-T674I-V5/HIS

    Plasmid
    #234756
    Purpose
    Expression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinib
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    T674I substitution
    Promoter
    CMV
    Available Since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    234756-plasmid-map-sequence-id-468865

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  19. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pNJP

    Plasmid
    #115494
    Purpose
    Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d
    Insert
    NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Human, Synthetic, Mouse)
    Tags
    iRFP, mCherry, and mKO
    Expression
    Mammalian
    Mutation
    mCherry S227F, mCherry G229R, mKO V211G
    Promoter
    CAG
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115494-plasmid-map-sequence-id-231521

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  21. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available Since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112060-plasmid-map-sequence-id-216934

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  23. pcDNA3.1-KozATG-dH6.2-2XG4S-actin

    Plasmid
    #73263
    Purpose
    Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)
    Depositor
    Marcel Bruchez
    Article
    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.
    Insert
    KozATG-dH6.2-2XG4S-actin (ACTB Human, Synthetic)
    Tags
    The FAP and actin are fused with 2 copies of a G4…
    Expression
    Mammalian
    Mutation
    The dH6.2 FAP has one cysteine in each domain cha…
    Promoter
    CMV
    Available Since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73263-plasmid-map-sequence-id-137070

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  24. Drd1a->M71-IRES-tauGFP ACNF TV

    Plasmid
    #105073
    Purpose
    Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette
    Depositor
    Peter Mombaerts
    Article
    Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.
    Insert
    Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)
    Use
    Mouse Targeting
    Mutation
    Drd1a coding sequence followed by IRES-tauGFP ACNF
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    105073-plasmid-map-sequence-id-204136

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  37. NMUR2-DuET

    Plasmid
    #213350
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    NMUR2 (NMUR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213350-plasmid-map-sequence-id-430914

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  40. pCMV-MtAcKRS (human-opti)

    Plasmid
    #164195
    Purpose
    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion
    Depositor
    Tao Liu
    Article
    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2
    Insert
    MtAcKRS-478(human-opti)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    D76G; L325M; L329I; Y330F; L333A; C372F
    Promoter
    CMV
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164195-plasmid-map-sequence-id-325763

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  45. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid
    #101347
    Purpose
    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.
    Depositor
    Jeremy Luban
    Article
    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:
    Insert
    NL4-3
    Use
    Lentiviral
    Mutation
    delta Tat
    Available Since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    101347-plasmid-map-sequence-id-194257

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Showing: 11451 - 11500 of 12455 results