We narrowed to 22,279 results for: fus

1. pCAG-Flpe:GFP

   Plasmid

   #13788

   Purpose

   Mammalian expression of enhanced form of Flp recombinase fused to GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Flpe-GFP fusion protein
   

   Use

   Flp/frt

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTAT-HA

   Plasmid

   #35612

   Depositor

   Steven Dowdy

   Article

   Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

   Type

   Empty backbone

   Use

   Tags

   6xHIS, HA, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. HaloTag-G protein beta-1

   Plasmid

   #67016

   Purpose

   G protein beta-1 tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-G protein beta-1 (GNB1 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-A96 ELP

   Plasmid

   #67856

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residue

   Depositor

   Andrew MacKay

   Insert

   GFP fused to ELP (VPGAG) repeated 96 times
   

   Use

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG4

   Plasmid

   #70115

   Purpose

   Tn7-based lux reporter for promoter cloning in bacteria

   Depositor

   Thomas Lewis

   Article

   Glassing et al J Microbiol Methods. 2015 Sep 1;118:75-7

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLJSRSF7

   Plasmid

   #64693

   Purpose

   His-MBP-Smt3-NpuDnaEN102-CBD

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Type

   Empty backbone

   Use

   Tags

   CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG:myr-Venus

   Plasmid

   #32602

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Rhee et al Genesis. 2006 Apr;44(4):202-18.

   Insert

   myr-Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

8. SA-betageo

   Plasmid

   #21709

   Depositor

   Philippe Soriano

   Article

   Friedrich et al Genes Dev. 1991 Sep . 5(9):1513-23.

   Insert

   SAbgeo
   

   Use

   Mouse gene trapping

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCARSF63

   Plasmid

   #64695

   Purpose

   H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Type

   Empty backbone

   Use

   Tags

   CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJSRSF3

    Plasmid

    #64692

    Purpose

    His-GST-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Use

    Tags

    CBD, GST, HisTag, NpuDnaE-N102, and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSABEd

    Plasmid

    #133968

    Purpose

    Expresses ABEd CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    ABEd
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKTol2P-PTK

    Plasmid

    #85599

    Purpose

    Tol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSABEn

    Plasmid

    #133969

    Purpose

    Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    ABEn
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-I48 ELP

    Plasmid

    #67857

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residues

    Depositor

    Andrew MacKay

    Insert

    GFP fused to ELP (VPGIG) repeated 48 times
    

    Use

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBNJ24.6

    Plasmid

    #66873

    Purpose

    for transposon tagging with a fluorescent protein

    Depositor

    Thomas Hughes

    Article

    Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

    Insert

    eGFP in a transposon
    

    Use

    Source for a tn7 transposon

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    none

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pSCBE3-HF

    Plasmid

    #133967

    Purpose

    Expresses HF-BE3 CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    HF-BE3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGGW_SM_BamHI

    Plasmid

    #49315

    Purpose

    Subcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insert

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBNJ28.2

    Plasmid

    #66874

    Purpose

    For transposon tagging with YFP

    Depositor

    Thomas Hughes

    Article

    Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

    Insert

    eYFP in a transposon
    

    Use

    Source for a tn7 transposon

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHSG-PCNA1

    Plasmid

    #66125

    Purpose

    S. solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

    Insert

    DNA polymerase sliding clamp 1 (SSO_RS01995 Sulfolobus solfataricus P2)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPTn2P-PTK

    Plasmid

    #85604

    Purpose

    Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Passport transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHSG-PCNA3

    Plasmid

    #66127

    Purpose

    S. solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

    Insert

    DNA polymerase sliding clamp 3 (pcn Sulfolobus solfataricus P2)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHSG-PCNA2

    Plasmid

    #66126

    Purpose

    S. solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

    Insert

    DNA polymerase sliding clamp 2 (SSO_RS05185 Sulfolobus solfataricus P2)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBNJ26.3

    Plasmid

    #66876

    Purpose

    For CFP tagging via transposon insertion.

    Depositor

    Thomas Hughes

    Article

    Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

    Insert

    eCFP in a transposon
    

    Use

    Source for a tn7 transposon

    Tags

    eCFP

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGGEV_5'_Linker

    Plasmid

    #49291

    Purpose

    Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGGEV_4'_XcmI-LacZ

    Plasmid

    #49305

    Purpose

    Golden Gate entry vector at position 4, LacZ, final entry vector for the assembly

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGGEV_+1_Linker

    Plasmid

    #49280

    Purpose

    Golden Gate entry vector at position 1,small linker insert, plus 5' ATG

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGGEV_3'_Linker

    Plasmid

    #49289

    Purpose

    Golden Gate entry vector at position 3, small linker insert, final entry vector for the assembly

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDsRed1-CMV-Rat Nurr1-DsRed1-pA

    Plasmid

    #233275

    Purpose

    To Express a Rat Nurr1-DsRed fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski, Glenn Schafe

    Insert

    rat Nurr1 DsRed fusion (Npas1 Rat)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    P2A

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Venus-79aa-ActA-pEGFP-C1

    Plasmid

    #166763

    Purpose

    To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (Listeria monocytogenes) (actA Synthetic, Listeria monocytogenes)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRSETB BS2mT4L1

    Plasmid

    #141203

    Purpose

    Chimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.

    Depositor

    Francesca Paradisi

    Article

    Planchestainer et al Catalysts 2018, 8(1), 40

    Insert

    BS2m-T4L1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E11Q

    Promoter

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge