We narrowed to 15,879 results for: NOL

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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NG-ABE8e

Plasmid

#138491

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (NG)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pT2-CAG-fGFP

Plasmid

#108714

Purpose

Transposon donor plasmid for long-term expression of membrane targetted GFP

Depositor

Madeline Lancaster

Article

Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

Insert

EGFP

Tags

6xHis and farnesylation signal

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR01-FnCpf1

Plasmid

#176529

Purpose

Nuclease Cpf1 adapted for Aspergillus species

Depositor

Kusol Pootanakit

Article

Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

Insert

FnCpf1

Use

CRISPR; Fungal expression

Promoter

TEF1

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(C)-SH2

Plasmid

#46469

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(C)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 656-766

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET-(-30)GFP-9xGGS-Cre-6xHis

Plasmid

#62372

Purpose

Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP-9xGGS-Cre-6xHis

Tags

(-30)GFP and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin TS

Plasmid

#68127

Purpose

nesprin tension sensor

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

mouse nesprin mini 2G (Syne2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT-EGFP-donor fw copy

Plasmid

#22074

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CIRBP

Plasmid

#67055

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CIRBP (CIRBP Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-PEmax-P2A-Puro

Plasmid

#228890

Purpose

Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Inserts

PEmax
Puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-NLS-6xHis

Plasmid

#62933

Purpose

Expression of Cas9-NLS-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9-NLS-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEdit

Plasmid

#232355

Purpose

The pEdit series plasmids are derived from the pTarget series plasmids by inserting homologous recombination arms targeting the gene of interest, which enables gene knockout or gene insertion at the t

Depositor

Yujie Cai

Article

Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-

Insert

sgRNA

Use

CRISPR

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IKKe T7

Plasmid

#27238

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon (IKBKE Human)

Tags

T7

Expression

Mammalian

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
enAsABE8e

Plasmid

#138506

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-dAsCas12a (En)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max

Plasmid

#138155

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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MS2-epegRNA-Dnmt1

Plasmid

#211372

Purpose

Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MS2-epegRNA

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Expression

Worm

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pMV_AA088

Plasmid

#216104

Purpose

Knockout, EF1a-driven Cas9 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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EGFR-mEGFP

Plasmid

#203774

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Gupta et al J Lipid Res. 2022 Jun;63(6):100220. doi:

Insert

EGFR (EGFR Human)

Tags

mEGFP

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSL2621 (pCas8_Vch)

Plasmid

#200893

Purpose

Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas8

Tags

NLS

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2622 (pCas7_Vch)

Plasmid

#200894

Purpose

Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas7

Tags

NLS

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2669 (pTnsABf_Vch)

Plasmid

#200897

Purpose

Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsA-TnsB

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB6364

Plasmid

#106151

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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