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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 26,454 results for: CAL

Showing: 9321 - 9360 of 26454 results

  1. pCDNA3-2xMARS-PLD

    Plasmid
    #205237
    Purpose
    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF
    Depositor
    Jeremy Baskin
    Inserts
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    Use
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205237-plasmid-map-sequence-id-404742

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  13. Rif1-Halo HRD

    Plasmid
    #207081
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207081-plasmid-map-sequence-id-432324

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  14. RNF169-Halo HRD

    Plasmid
    #207083
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207083-plasmid-map-sequence-id-432319

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  15. Halo-SHLD2 HRD

    Plasmid
    #207078
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    207078-plasmid-map-sequence-id-431782

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  21. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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  22. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  29. pUAS-kkv-OH

    Plasmid
    #138956
    Purpose
    Used to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv with C terminal epitope tag (kkv Fly)
    Use
    Tags
    ollas and his-6 added to C terminus of protein co…
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138956-plasmid-map-sequence-id-274764

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  30. pHD-DsRed-kkv-3::NG

    Plasmid
    #138960
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    3' end of kkv and neon green (kkv Fly)
    Use
    An editing vector for drosophila
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138960-plasmid-map-sequence-id-274768

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  38. FB396

    Plasmid
    #203632
    Purpose
    TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).
    Depositor
    Jose Marcos
    Article
    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1
    Insert
    R1:G1ab.1:mPAF:Luc:TtrpC
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203632-plasmid-map-sequence-id-405888

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  39. FB397

    Plasmid
    #203633
    Purpose
    TU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).
    Depositor
    Jose Marcos
    Article
    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1
    Insert
    R1:G1abc.3:mPAF:Luc:TtrpC
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203633-plasmid-map-sequence-id-405886

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Showing: 9321 - 9360 of 26454 results