We narrowed to 23,191 results for: his

Showing: 9361 - 9400 of 23191 results

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pCellFree_G03 WWP1

Plasmid

#67125

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WWP1 (WWP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(T21N)-RagC

Plasmid

#99644

Purpose

Bacterial expression of RagA(T21N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

T21N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-STX6

Plasmid

#31581

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

syntaxin 6 (STX6 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pTKhc-yibDp-GFP-cp6

Plasmid

#134938

Purpose

Low phosphate inducible circular permutation of GFP

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPcp6

Tags

His tag

Expression

Bacterial

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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PB-NLS-ZapCV2-ALA

Plasmid

#203665

Purpose

Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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hCdc20 (1-120)

Plasmid

#8509

Depositor

Joan Ruderman

Article

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hCdc20 (1-120) (CDC20 Human)

Use

Retroviral

Tags

His

Expression

Mammalian

Mutation

Cdc20 N-terminus. Dominant Negative mutant.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pET15b/GgVcl 1-1066 (full length)

Plasmid

#46171

Depositor

Susan Craig

Article

Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

Insert

Vcl (VCL Chicken)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(D181N)

Plasmid

#99643

Purpose

Bacterial expression of RagA-RagC(D181N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

D181N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 POLD1

Plasmid

#67128

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

POLD1 (POLD1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G02

Plasmid

#67136

Purpose

Gateway destination vector for cell-free expression with C-term 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GCaMP2-actin

Plasmid

#18928

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2-Actin (ACTB Chicken)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pET15b/BOA

Plasmid

#82041

Purpose

For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

Depositor

Angela Gronenborn

Article

Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

Insert

Burkholderia oklahomensis agglutinin

Tags

6x His

Expression

Bacterial

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFAP2B

Plasmid

#67092

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAP2B (TFAP2B Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMCSG7-D16A-NmeCas9

Plasmid

#71476

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-D16A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSG7-H588A-NmeCas9

Plasmid

#71477

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-H588A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

H588A

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR C (709-1)

Plasmid

#17429

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMS17: pTwinStrep-SUMO-AvTnsD

Plasmid

#168150

Purpose

AvTnsD expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsD

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS15: pTwinStrep-SUMO-AvTnsB

Plasmid

#168148

Purpose

AvTnsB expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsB

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS16: pTwinStrep-SUMO-AvTnsC

Plasmid

#168149

Purpose

AvTnsC expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsC

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS14: pTwinStrep-SUMO-AvTnsA

Plasmid

#168147

Purpose

AvTnsA expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST TnsA

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET30 GLTP

Plasmid

#170733

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

H759A

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pHCKan-INSyibp-Nef

Plasmid

#134593

Purpose

Low Phosphate inducible Nef

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

HIV Nef protein

Tags

His tag

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-Nanog

Plasmid

#13988

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

Nanog (Nanog Mouse)

Tags

His

Expression

Bacterial

Mutation

None

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA2

Plasmid

#145370

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Tags

His and mCherry

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDC20

Plasmid

#131366

Purpose

expression of MEG-3 (aa1-862) in C. elegans

Depositor

Geraldine Seydoux

Article

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa1-862) (meg-3 Nematode)

Tags

His

Expression

Bacterial

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBTRCk-pVhb-maACR

Plasmid

#215651

Purpose

pBBR1 Origin, KanR, with maACR gene under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

acyl-CoA reductase

Tags

Maltose binding protein and His tag

Expression

Bacterial

Promoter

Vhb

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP E17TAG/Q157TAG

Plasmid

#68299

Purpose

E17/Q157 TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP E17TAG/Q157TAG

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSir1

Plasmid

#107447

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sir1 (sir1 Fission Yeast)

Tags

V5-His

Expression

Yeast

Mutation

H523R, A750T

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB328

Plasmid

#113672

Purpose

expresses Ulp1_R4 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R4

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-ENTR B (708-1)

Plasmid

#17428

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame B

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FRB-LplA(W37V)

Plasmid

#35608

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37V)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB_init_Sb92

Plasmid

#159424

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb92

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pETM41-EcPPXc

Plasmid

#38329

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pYTSG

Plasmid

#159419

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Yeast

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pAC-Cyc

Plasmid

#33156

Depositor

Michael Rosbash

Article

Kadener et al PLoS Biol. 2008 May 20;6(5):e119.

Insert

Cyc (cyc Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 23,191 results for: his