We narrowed to 13,228 results for: 109

1. pUPD2 RDF ( GB1498)

   Plasmid

   #160579

   Purpose

   Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSMP-DNMT1_1

   Plasmid

   #36382

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pX459-HypaCas9-mR2-TUBA1B_sgRNA

   Plasmid

   #183888

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCYC1pr-GNSI (LEU)

   Plasmid

   #111450

   Purpose

   GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

   Depositor

   Alexey Merz

   Article

   Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

   Inserts

   CYC1 Promoter (CYC1 Budding Yeast)
   Nyv1 Cytosolic Domain (aa 6-230)
   Snc1 Transmembrane Domain (TMD)
   SUC2 CDS
   SUC2 terminator
   

   Use

   Tags

   mGFP5

   Expression

   Yeast

   Mutation

   M109I (Naturally occurring SNP) and The first 21 …

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR4 PARP2 E614Q

   Plasmid

   #192545

   Purpose

   pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   changed Glu 614 to Gln

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YFP-Rpp40-C1

   Plasmid

   #134549

   Purpose

   Expresses YFP-Rpp40 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Rpp40 (RPP40 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS3-MT-BX/p14ARF

   Plasmid

   #78761

   Purpose

   To overexpress p14ARF in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

   Insert

   ARF (CDKN2A Human)
   

   Use

   Tags

   6xMYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

8. gh113

   Plasmid

   #106783

   Purpose

   expression of gRNA targeting B4GALNT1

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   B4GALNT1 (B4GALNT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCW57.1-pDUXChd:hDUX4ctd-ca

   Plasmid

   #198237

   Purpose

   Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

   Depositor

   Stephen Tapscott

   Article

   Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

   Insert

   DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon altered

   Promoter

   TRE

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid

    #121833

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Cx43-Myc

    Plasmid

    #60693

    Purpose

    mammalian expression of Myc-tagged rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTE4560

    Plasmid

    #107526

    Purpose

    Expresses As crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gh88

    Plasmid

    #106759

    Purpose

    expression of gRNA targeting FUT4

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT4 (FUT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Frt-hspB9

    Plasmid

    #63100

    Purpose

    Expression of HSPB9 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb9 (HSPB9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-BAP1-S172R-GFP

    Plasmid

    #78918

    Purpose

    Mammalian expression of BAP1 with S172R and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    S172R

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pRF+275Dux4

    Plasmid

    #21293

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX 4 (DUX4 Human, DUX 4)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

17. LXSN-Cx43

    Plasmid

    #60692

    Purpose

    retroviral expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pUPD2 PhiC31 integrase (GB1496)

    Plasmid

    #160578

    Purpose

    Streptomyces phage PhiC31 integrase, complete CDS

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_6HT-TBP6.7(R49A)

    Plasmid

    #112726

    Purpose

    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R49A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R49A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW1883

    Plasmid

    #154331

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    ttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-BAP1-C91W-GFP

    Plasmid

    #78913

    Purpose

    Mammalian expression of BAP1 with C91W and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    C91W

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pJW1947

    Plasmid

    #154339

    Purpose

    Enhancer reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    pes-10delta::SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDIV494

    Plasmid

    #177701

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-C3-PLK4-S305E-3xFLAG

    Plasmid

    #69840

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S305E

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC19-G2ZYP2

    Plasmid

    #103110

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T-1-Dicer

    Plasmid

    #171543

    Purpose

    Encodes a Dicer fragment extending from from R1841 to the C-terminus

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    DICER1 (DICER1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRD398

    Plasmid

    #163105

    Purpose

    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mCherry_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Cry2 mCherry PGK-1 in pcDNA3.1

    Plasmid

    #64214

    Purpose

    mCherry fused between Cry2 and PGK-1 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    PGK1 (PGK1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

    Plasmid

    #69841

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S285A/T289A

    Promoter

    CMV

    Available since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSMP-Ehmt1_4

    Plasmid

    #36338

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Ehmt1 (EHMT1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pBW_0001

    Plasmid

    #102833

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRD408

    Plasmid

    #178199

    Purpose

    Expression of H-NS from its natural promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + hns (hns Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hns promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCS2-mqgDUX4

    Plasmid

    #21176

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pUC19-Q927P4

    Plasmid

    #103131

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. myc-hRAD18 dSAP

    Plasmid

    #68830

    Purpose

    Expresses c-myc-tagged human RAD18 deleting SAP domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pTER E2F3shRNA human

    Plasmid

    #66885

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 3 (E2F3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

37. myc-hRAD18 dC2

    Plasmid

    #68831

    Purpose

    Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pSMP-Ehmt1_1

    Plasmid

    #36335

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Ehmt1 (EHMT1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

    Plasmid

    #160616

    Purpose

    TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS3-MT-BX/ARF1-64

    Plasmid

    #78762

    Purpose

    To overexpress ARF1-64 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    1-64

    Promoter

    CMV IE94

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

41. pCS3-MT-BX/ARF65-132

    Plasmid

    #78763

    Purpose

    To overexpress ARF65-132 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    65-132

    Promoter

    CMV IE94

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

42. pCAG-F-API5

    Plasmid

    #157662

    Purpose

    Expresses API5 in mammalian cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRD190

    Plasmid

    #163100

    Purpose

    Expression of mEos3.2 in eukaryotes to stain the nuclear compartment

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mEos3.2_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Frt-hspB6

    Plasmid

    #63097

    Purpose

    Expression of HSPB6 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb6 (HSPB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDEST-DHFR F[3]-C (LEU2)

    Plasmid

    #177796

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[3]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. gh266

    Plasmid

    #106865

    Purpose

    expression of gRNA targeting ALG12

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ALG12 (ALG12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. gh152

    Plasmid

    #106822

    Purpose

    expression of gRNA targeting GLT8D2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    GLT8D2 (GLT8D2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEGFPN1-N-gamma-tubulin (1-333)

    Plasmid

    #87858

    Purpose

    Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Fragment from aa 1 to 333

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. myc-hRAD18 d6BD

    Plasmid

    #68832

    Purpose

    Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

50. pCCL-PGK-SPdCas9-BFP-EGFP

    Plasmid

    #66821

    Purpose

    dCas9 fused to BFP and EGFP

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-EGFP
    

    Use

    Tags

    dSpCas9-BFP-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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