We narrowed to 26,754 results for: STI

1. α-actinin-4-M522-sstFRET(Venus/Cerulean) in pUNO1

   Plasmid

   #190799

   Purpose

   Imaging of force-sensitive α-actinin-4 in live cells

   Depositor

   Vivian Tang

   Article

   Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

   Insert

   α-actinin-4-Venus-Cerulean-522 (ACTN4 Synthetic, Human)
   

   Tags

   cerulean and venus

   Expression

   Mammalian

   Promoter

   hEF1-HTLV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_iSH2-pMag(3x)-iRFP

   Plasmid

   #67304

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   iSH2-pMag(3x)-iRFP (PIK3R1 Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TetON-Bmpr1A-ca

   Plasmid

   #217536

   Purpose

   AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   constitutively active BMP receptor 1a (BMPR1A Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   Tet operon (TetO)

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6-AMPK alpha1 (1-312)

   Plasmid

   #162131

   Purpose

   Expression vector for constitutively active AMPK alpha 1

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

   Insert

   AMPK alpha1 (1-312) (PRKAA1 Rat)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROPseq-Guide-BSD

   Plasmid

   #216123

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-TSF-TEVcs-ULK1K46I

   Plasmid

   #171417

   Purpose

   For the purifcation of human ULK1 kinase dead complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ULK1 (ULK1 Synthetic)
   

   Expression

   Mammalian

   Mutation

   kinase dead

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MiniCoopR U6:gRNA spred1, mitfa:Cas9

   Plasmid

   #118843

   Purpose

   Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   spred1 gRNA (spred1 Zebrafish)
   

   Use

   CRISPR; Tol2

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRi(v1)-Blast

   Plasmid

   #170067

   Purpose

   Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

   Plasmid

   #101209

   Purpose

   Bacterial expression plasmid for SpCas9-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A/Q926A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

    Plasmid

    #40809

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S218E, S222D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

11. IKK2-EGFP

    Plasmid

    #111195

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

    Insert

    IKKbeta (IKBKB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT C-His Ana GvpC in pET28a

    Plasmid

    #85732

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-6P-acatenin

    Plasmid

    #186458

    Purpose

    Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK351

    Plasmid

    #129696

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120034435
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKIIa-Flpo

    Plasmid

    #183535

    Purpose

    INTRSECT

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Has Service

    AAV8

    Insert

    Flpo
    

    Use

    AAV

    Mutation

    None

    Promoter

    CaMKIIa

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-AsCpf1-Blast

    Plasmid

    #84750

    Purpose

    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    AsCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-Climp63(1–336)

    Plasmid

    #186957

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180266

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1A-C1A-EYFP

    Plasmid

    #61155

    Purpose

    Diacylglycerol (DAG) reporter

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C1A from PKCG amino acids 26-89

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-FOXO3a TM-ER

    Plasmid

    #8353

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, mouse estrogen receptor (G525…

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pRExT2A-NT-PmNG

    Plasmid

    #232200

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1_iSH2-pMagFast2(3x)-iRFP

    Plasmid

    #67298

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    iSH2-pMagFast2(3x)-iRFP (PIK3R1 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. HRAS

    Plasmid

    #55653

    Purpose

    expression of mouse HRAS

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    HRAS (Hras Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. tdTomato-KIF1A

    Plasmid

    #102850

    Purpose

    Overexpresses KIF1A and cell fill TdTomato

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A (Kif1a Rat)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. bArr1-V5

    Plasmid

    #201492

    Purpose

    Expression of the beta-arrestin1 containing a C-terminal V5 tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr1-V5 (ARRB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #50942

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Tobias Rose, Mark Huebener, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. HypaCas9

    Plasmid

    #138557

    Purpose

    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKLV2-EF1a-Cas9GFP-W

    Plasmid

    #200100

    Purpose

    Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOPS0381

    Plasmid

    #133231

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pRK5-myc-Miro1 P13V

    Plasmid

    #47889

    Purpose

    Expresses myc tagged Miro1 P13V constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 P13V (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    P13V, constitutively active mutant

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA3 deltaN47 Beta-catenin

    Plasmid

    #19287

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-47

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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32. pET29a-hNABP1

    Plasmid

    #128306

    Purpose

    Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HA-FOXO3a TM-ER-delta DB

    Plasmid

    #8354

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, ER (G525R), delta DB (deletio…

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pLUEF1 Luc2-IRES-GFP

    Plasmid

    #224467

    Purpose

    Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

    Depositor

    Robert Kerbel

    Article

    Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

    Insert

    Luciferase
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ER-NFAST

    Plasmid

    #233596

    Purpose

    Expression of NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    NFAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR-mGFP-SNX9

    Plasmid

    #223632

    Purpose

    Lentiviral expression of human SNX9 with mGFP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SNX9 (SNX9 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mGFP

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-MLC2(CA)-IRES-GFP

    Plasmid

    #133928

    Purpose

    Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EGFP and MLC2(CA) (MYL2 Human)
    

    Expression

    Mammalian

    Mutation

    MLC2 T18D, S19D

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMXs-puro Vps34-GFP

    Plasmid

    #38268

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid

    #163928

    Purpose

    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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