170,204 results
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Plasmid#70128PurposeNegative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loopDepositorInsertkissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
UseSynthetic BiologyTagsNoneExpressionBacterialPromotertacAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWP017
Plasmid#198780PurposeDrives specific expression in the ASI neuronsDepositorInsertPgpa-4-GAL4-SK(DBD)-VP64
ExpressionWormAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIG-836_NY-ESO-1_TCR_FAS/OX40
Plasmid#207499PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertFAS/OX40, NY-ESO-1_TCR (FAS Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterPromoter1.7kb fabp6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLE1 N-ISCmut
Plasmid#160803PurposeExpress N-terminal ISC mutant POLE1DepositorInsertPOLE1 N-ISCmut (POLE Human)
UseRetroviralTags3xFLAGMutationshPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…Available SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SLAMF6
Plasmid#23396DepositorInsertSLAMF6 (SLAMF6 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RbE2Fmut
Plasmid#32391DepositorInsertRb promoter (Rb1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutated E2F binding site: TTTCCCGC --> TTTACATCAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Rb WT
Plasmid#32390DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
MUT
Plasmid#38872PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter hsa-let-7a-1
Plasmid#46670DepositorInserthsa-let-7a-1 (MIRLET7A1 Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-GTF2H1
Plasmid#23445DepositorInsertGTF2H1 (GTF2H1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Con/Fon DREADD Gq-mCherry
Plasmid#183532PurposeINTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND FlpDepositorInsertDREADD Gq-mCherry
UseAAVMutationNonePromoternEFAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro shPOLE1_1
Plasmid#160810PurposeExpress Dox repressible shPOLE1DepositorInsertshPOLE1_1
UseLentiviralAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-com-BoxB-vector
Plasmid#132556PurposeExpresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.DepositorInsertsdCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
Lambda N22-KRAB
sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
ExpressionMammalianAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4-UPRE-luc2P-Hygro
Plasmid#101788PurposeLuciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)DepositorInsert3xUPRE
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
mRuby-FYN-N-10
Plasmid#55863PurposeLocalization: Membrane Associated Kinase, Excitation: 558, Emission: 605DepositorAvailable SinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mPA-GFP-Paxillin-22
Plasmid#57153PurposeLocalization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertPaxillin
TagsmPA-GFPExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
SIN-cPPT-PGK-mitoGO-ATeam2-WPRE
Plasmid#245083PurposeMitochondrial ATP biosensorDepositorInsertmitoGO-ATeam2
UseLentiviralExpressionMammalianPromoterMouse PGKAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
palm-Bassoon-GFP
Plasmid#250161PurposeEncodes a version of rat Bassoon with a C-terminal GFP-tag and with a palmitoylation consensus replacing the N-terminal 94 aa. For mammalian expression.DepositorInsertpalmitoylated Bassoon-GFP construct (N-terminal 94 aa replaced by palmitoylation consensus) (Bsn Rat)
ExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2026AvailabilityAcademic Institutions and Nonprofits only