We narrowed to 13,228 results for: 109
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Plasmid#192216PurposeMutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertI-F leader and I-F repeat construct not studied further in this paper (X1)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX2-IFl_Con-IFr
Plasmid#192217PurposeMutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertI-F leader and I-F repeat construct not studied further in this paper (X2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWT-IFl_WT-IFr
Plasmid#192207PurposeWildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertWildtype I-F Leader and wildtype I-F Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScr-IFl_WT-IFr
Plasmid#192208PurposeScrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertScrambled I-F Leader and wildtype I-F Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IFr
Plasmid#192209PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-F Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IEr
Plasmid#192210PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-E Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)
Plasmid#226884PurposeExpresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cellsDepositorInsertNaVAb
UseTagsMBPExpressionInsectMutationR4A, N49K, L109A, M116VPromoterPolyhedrinAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEIF3D-a11helixDel-sgEIF3D
Plasmid#222135PurposeEIF3D variant overexpression vector with sgRNA targeting eIF3DDepositorInserteIF3D (EIF3D Human)
UseLentiviralTagsBFP-P2AExpressionMutationT317E, N320E, H321APromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEIF3D-CtermDel-sgEIF3D
Plasmid#222125PurposeEIF3D variant overexpression vector with sgRNA targeting eIF3DDepositorInserteIF3D (EIF3D Human)
UseLentiviralTagsBFP-P2AExpressionMutationF527 truncationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEIF3D-a5helixDel-sgEIF3D
Plasmid#222133PurposeEIF3D variant overexpression vector with sgRNA targeting eIF3DDepositorInserteIF3D (EIF3D Human)
UseLentiviralTagsBFP-P2AExpressionMutationD249Q, V262I, Y263APromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_SOX2_HDR
Plasmid#163752PurposegRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.DepositorInsertgRNA_SOX2 (SOX2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR104
Plasmid#185034Purpose400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationA74 and A76 deletion of rpmE 5'UTR, Only fir…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR105
Plasmid#185035Purpose400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationG52 and G79 deletion of rpmE 5'UTR, Only fir…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR107
Plasmid#185037Purpose400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationAGA74-76GAG mutation of rpmE 5'UTR, Only fir…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR110
Plasmid#185039Purpose400 nt l31 promoter region, l31 5'UTR G62 and G67 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationG62 and G67 deletion of rpmE 5'UTR, Only fir…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-INCENP∆cen
Plasmid#184044PurposeExpresses H2B-GFP fused to INCENP∆cenDepositorInsertINCENP (INCENP Human)
UseTagsGFP and H2BExpressionMammalianMutationTruncated to 1-862PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL43C
Plasmid#218278PurposeIntroduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL4A
Plasmid#218277PurposeIntroduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL3C
Plasmid#218275PurposeIntroduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCAM-BirA*-HA
Plasmid#214472PurposeLentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.DepositorInsertMCAM (MCAM Human)
UseLentiviral; GatewayTagsBirA(R118G) and HA tagExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1-Cdc45-6His
Plasmid#211379Purposebacterial expression of human Cdc45 C-terminally fused to 6xHis-TagDepositorInsertCdc45 (CDC45 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR
Plasmid#211820PurposeLentiviral vector expressing a dead GFP adenine base editing reporterDepositorInsertdead GFP reporter
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoter and SV40 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ08-pAAVsc.U6-tracrRNA
Plasmid#211816PurposeAAV vector expressing tracrRNA under U6 promoterDepositorInsertU6-tracrRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A-A28C_R
Plasmid#200185PurposeWT PA14 I-F Leader and G1A-T2A-A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
UseTagsExpressionBacterialMutationG1A-T2A-A28C in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-457
Plasmid#200213PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 H25A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-458
Plasmid#200214PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 E184A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-459
Plasmid#200215PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 K214A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-460
Plasmid#200216PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-461
Plasmid#200217PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-462
Plasmid#200218PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E, R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bn CAAX pcDNA3
Plasmid#206088PurposeCav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motifDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-626_pGEX6P1
Plasmid#209790PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
UseTagsGSTExpressionBacterialMutationaa 544-626 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-509
Plasmid#209800PurposeBacterial expression of human LARP1 La-moduleDepositorInsertLARP1 La-module (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-509 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-410
Plasmid#209801PurposeBacterial expression of human LARP1 LaM domainDepositorInsertLARP1 LaM (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-410 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMX1463
Plasmid#208805PurposeExpresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycinDepositorInsertFRB (CRP Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-2
Plasmid#210136Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-1
Plasmid#210135Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 mePOU-POU
Plasmid#206392PurposeExpresses the DNA binding domain of the ePOU with a 6xHis tag for protein purificationDepositorInsertePOU
UseTags6xHisExpressionBacterialMutationPOU DNA binding domain onlyPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 hU6 ACTB sgRNA
Plasmid#206270PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNADepositorInsertACTB sgRNA
UseCRISPR; Multimate/gateway entr 2TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 hU6 HEK3 PegRNA
Plasmid#206277PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNADepositorInsertHEK3 PegRNA
UseCRISPR; Multimate/gateway entr 2TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 polH aeBlue polH VSV-G
Plasmid#206278PurposeENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.DepositorInsertaeBlue, VSV-G
UseMultimate/gateway entr 3TagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_B
Plasmid#206135PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_A
Plasmid#206134PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LYK5_At-3xHA
Plasmid#202192PurposeExpress Arabidopsis thaliana LYK5 gene under UBQ10 promoterDepositorInsertLYSM-CONTAINING RECEPTOR-LIKE KINASE 5 (LYK5 Mustard Weed)
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-BS-AtMIR390a-B/c
Plasmid#199559PurposeGATEWAY-compatible entry vectorDepositorInsertBasal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-S13S2DBD
Plasmid#200196PurposeExpression of Sox13 with Sox2 DBDDepositorUseTagsHaloExpressionMammalianMutationSox13(1-1242); Sox 2 (126-333); Sox13 (1449-1839)PromoterEF_1alphaAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-NL63-Protein3
Plasmid#168923PurposeGateway-compatible Entry vectorDepositorInsertHCoV-NL63-Protein3 (HCNV63gp3 )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-Spike
Plasmid#168891PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-Spike (S )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2567
Plasmid#142328PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF654 (ZNF654 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only