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Addgene

We narrowed to 33,634 results for: CaS;

Showing: 11901 - 11950 of 33634 results
  1. pAAV-CMV-dSa VP64 Afp

    Plasmid
    #99697
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pBH504

    Plasmid
    #215782
    Purpose
    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene
    Depositor
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bt1311, P(O1), and P1
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. SOX2-3'gRNA

    Plasmid
    #215543
    Purpose
    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in
    Depositor
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid
    #215544
    Purpose
    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
    Depositor
    Insert
    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 11901 - 11950 of 33634 results