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We narrowed to 18,759 results for: nls

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Showing: 9641 - 9680 of 18759 results

  1. pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #196545
    Purpose
    Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu
    Publication
    DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Surface marker and Citrine reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196545-plasmid-map-sequence-id-384575

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  5. pLV-REPORT(EF1a)-TTN

    Plasmid
    #172327
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172327-plasmid-map-sequence-id-354684

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  7. dpYPQ230-D156R-CBE2

    Plasmid
    #195368
    Purpose
    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    hA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
    Use
    CRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…
    Expression
    Plant
    Promoter
    ZmUBI1
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195368-plasmid-map-sequence-id-392913

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  8. PC1-29-9-2_GAL4-VHL

    Plasmid
    #200434
    Purpose
    Expresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin Ligase
    Depositor
    Xue Gao
    Publication
    Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4-VHL
    Tags
    GAL4, VHL
    Expression
    Mammalian
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200434-plasmid-map-sequence-id-393109

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  13. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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  14. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid
    #196086
    Purpose
    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting lncRNA NORAD
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Cloned using SapI sites - destroyed after inserti…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196086-plasmid-map-sequence-id-388997

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  17. pICH-nuc-roGFP2-GPXC71S

    Plasmid
    #191760
    Purpose
    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758
    Depositor
    Philip Mullineaux
    Publication
    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236
    Insert
    nuc-roGFP2-GPXC71S
    Use
    Binary agrobacterium ti plasmid
    Expression
    Plant
    Mutation
    GPX has no peroxidatic Cysteine 71, which is repl…
    Promoter
    CaMV 35S
    Available since
    March 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191760-plasmid-map-sequence-id-378856

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  21. pCMV-PE2-p53DD

    Plasmid
    #196582
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD
    Depositor
    Ting Zhou
    Publication
    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-mp53DD
    Expression
    Mammalian
    Mutation
    Δ40-903
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196582-plasmid-map-sequence-id-386782

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  22. FUGW-3xHA-KCNJ6-mCherry

    Plasmid
    #197032
    Purpose
    Express 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, from UbiC promoter
    Depositor
    Ronald Hart
    Insert
    KCNJ6 (KCNJ6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    197032-plasmid-map-sequence-id-386375

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  32. Chr1q_Cassette-Integration_gRNA

    Plasmid
    #195126
    Purpose
    gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration
    Depositor
    Jason Sheltzer
    Publication
    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Chr1q gRNA
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195126-plasmid-map-sequence-id-383930

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  33. pX459-sgRNA2-RPB1-N-term

    Plasmid
    #195111
    Purpose
    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).
    Depositor
    Job Dekker
    Publication
    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against first exon of RPB1 (N-terminal)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195111-plasmid-map-sequence-id-383881

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  37. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Publication
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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  38. pSFFV-dNme2Cas9

    Plasmid
    #193190
    Purpose
    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)
    Depositor
    Erik Sontheimer
    Publication
    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dNme2Cas9
    Tags
    NLS and NLS-3xHA-NLS
    Expression
    Mammalian
    Mutation
    H588A, D16A
    Promoter
    SFFV
    Available since
    Jan. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    193190-plasmid-map-sequence-id-381070

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  40. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid
    #192939
    Purpose
    FlpO-based digitizer circuit driven by AP1 promoter
    Depositor
    Wilson Wong
    Publication
    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    Dec. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192939-plasmid-map-sequence-id-380399

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Showing: 9641 - 9680 of 18759 results