We narrowed to 15,070 results for: NTS

Showing: 7441 - 7470 of 15070 results

Results per page:

Sort results by:

lentiGuideFE-mSG-Puro

Plasmid

#226522

Purpose

Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

Depositor

Neville Sanjana

Article

Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

Insert

hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro

Use

CRISPR and Lentiviral

Tags

mStayGold

Expression

Mammalian

Promoter

U6,EF1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABEmax-NRTH

Plasmid

#136922

Purpose

Expresses ABEmax-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MPRA_minCMV+GFP

Plasmid

#204866

Purpose

Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Insert

minCMV:GFP

Expression

Bacterial and Mammalian

Promoter

minimal CMV

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AY27_pU6.gRNA.CLYBL

Plasmid

#199238

Purpose

Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. pyogenes gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-E-Syt2

Plasmid

#66831

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX302 OAS1-V5 puro

Plasmid

#158641

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

OAS1 (OAS1 Human)

Use

Lentiviral

Tags

V5

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BE4max-NRTH

Plasmid

#136919

Purpose

Expresses BE4max-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-HLA-A2

Plasmid

#135504

Purpose

Mammalian expression of myc-tagged HLA-A*02:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T-E2-25K

Plasmid

#63572

Purpose

Expression of human Ubiquitin conjugating enzyme E2-25K

Depositor

Titia Sixma

Article

Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

Insert

UBE2-25K (UBA7 Human)

Tags

GST

Expression

Bacterial

Mutation

Isoform 1 (200 resiudes)

Promoter

tac

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pGWB502-omega

Plasmid

#74845

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS578_DD-dSpyCas9-mCherry-APEX2

Plasmid

#108570

Purpose

Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Insert

dSpyCas9

Use

CRISPR and Lentiviral

Tags

APEX2, Destabilization domains, Flag, and mCherry…

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIRECT_22C

Plasmid

#91135

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1_10xHis_MBP_TEV_WT-Cas9

Plasmid

#244825

Purpose

Bacterial expression of SpyCas9 (WT) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-HLA-A3

Plasmid

#135505

Purpose

Mammalian expression of myc-tagged HLA-A*03:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*03:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

Plasmid

#196971

Purpose

Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

Depositor

Leopold Parts

Article

Koeppel et al Nat Biotechnol. 2023 Feb 16. doi: 10.103

Inserts

Cas9-RT
rtTA3G-P2A-eGFP

Expression

Mammalian

Promoter

PGK and TREG3G

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-HLA-B8

Plasmid

#135512

Purpose

Mammalian expression of myc-tagged HLA-B*08:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*08:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HypaCas9

Plasmid

#138557

Purpose

Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N692A, M694A, Q695A, H698A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
cacnb2a (rat) in pMT2 vector

Plasmid

#107424

Purpose

Expresses Calcium channel beta2a auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

Insert

calcium channel beta2a auxillary subunit (Cacnb2 Rat)

Expression

Mammalian

Promoter

Ad MLP

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn1-eYFP

Plasmid

#117382

Purpose

An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-N-mEGFP-PARP7

Plasmid

#178004

Purpose

Expresses N-terminally mEGFP-tagged PARP7

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP7 (TIPARP Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHUE411

Plasmid

#62203

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

OsU3 and Ubi

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-GRAB_OT1.0

Plasmid

#185386

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Has Service

AAV1 and AAV9

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

hSyn

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE8e SpG Puro

Plasmid

#235044

Purpose

Adenosine base editor 8e* with SpG nicking Cas9 makes A > G edits

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

ABE8e, nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge