163,394 results

1. Hy_pMT Flag-URH49 WT

   Plasmid

   #110127

   Purpose

   Expresses Flag-tagged human URH49 (DDX39A)

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   URH49 (DDX39A Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   April 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP Cited2

   Plasmid

   #112246

   Purpose

   expression of EGFP-Cited2 in mammalian cells

   Depositor

   Simone Di Giovanni

   Insert

   Cited2 (CITED2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPM-D4 miR30-TRIM28 ts1

   Plasmid

   #115862

   Purpose

   TRIM28 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   TRIM28 shRNA (TRIM28 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-His-SCov2-nsp7

   Plasmid

   #154757

   Purpose

   Expression of SARS-CoV-2 nsp7

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

   Plasmid

   #228401

   Purpose

   Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   SREBP1 (SREBF1 Human)
   

   Use

   Tags

   mNeonGreen2x

   Expression

   Mammalian

   Mutation

   deleted N terminal activation domain (amino acids…

   Promoter

   EF1a

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0169

   Plasmid

   #141540

   Purpose

   Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SIX3 (SIX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSH69

   Plasmid

   #127702

   Purpose

   Prab-3::ssGFP1-10

   Depositor

   David M. Miller

   Article

   He et al Genetics. 2019 Apr 5. pii: genetics.119.

   Insert

   ssGFP-1/10
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-mNeptune2.5

   Plasmid

   #197197

   Purpose

   Expresses the protein of mNeptune2.5 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   mNeptune2.5
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4 Smad4

   Plasmid

   #16483

   Depositor

   Bert Vogelstein

   Article

   Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

   Insert

   Smad4 (SMAD4 Human)
   

   Use

   Tags

   Double HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

    Plasmid

    #85798

    Purpose

    donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    homology arms for knock-in into first intron

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-mito-ZapCY1

    Plasmid

    #58996

    Purpose

    Mitochondrial Zn-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Park et al ACS Chem Biol. 2012 Aug 10.

    Insert

    mito-ZapCY1 high affinity Zn(II) sensor
    

    Use

    Tags

    See comment

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET-His-SCov2-nsp8

    Plasmid

    #154758

    Purpose

    Expression of SARS-CoV-2 nsp8

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSIIpuro-EPGN-ScNeo

    Plasmid

    #209899

    Purpose

    To monitor the status of Epigen, the plasmid encodes a recombinant Epigen fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EPGN-ScNeo (EPGN Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCN34e-ktrA-yfp

    Plasmid

    #227640

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    ktrA-yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-GalT-mCherry

    Plasmid

    #214271

    Purpose

    Mammalian expression of GalT-mCherry (Golgi marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    GalT(1-82)-mCherry (B4GALT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Stx4-pmCitrine

    Plasmid

    #92422

    Purpose

    Expression of syntaxin-4 C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. HMBP-3C-STAR.66-285

    Plasmid

    #100094

    Purpose

    expresses MBP-tagged STARD1 protein domain (residues 66-285)

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

    Insert

    STAR protein (STAR Human)
    

    Use

    Tags

    His-tagged MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFP.R268

    Plasmid

    #138180

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKM491-Flag-His

    Plasmid

    #109282

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Flag-His
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SI10-Brevican

    Plasmid

    #46300

    Depositor

    Gavin Wright

    Article

    Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

    Insert

    Brevican mouse monoclonal antibody (bcan Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only