159,238 results

1. pRM055 His-TEV-Cas9 6xNLS 0C

   Plasmid

   #196246

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CTGF-bio-His

   Plasmid

   #52028

   Purpose

   Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CTGF (CCN2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

   Plasmid

   #119946

   Purpose

   Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLD-puro-Cc-TARDBP-WT_VA

   Plasmid

   #141327

   Purpose

   Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

   Depositor

   Mohan Babu

   Article

   Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

   Insert

   TARDBP-WT (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST47-SAR1-GFP

   Plasmid

   #67409

   Purpose

   Mammalian expression of hSar1 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SAR1 (SAR1A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pkk223_Null

   Plasmid

   #213111

   Purpose

   pkk223 backbone plasmid

   Depositor

   Martin Horvath

   Article

   Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP PICH (Nigg CB62)

   Plasmid

   #41163

   Depositor

   Erich Nigg

   Article

   Baumann et al Cell. 2007 Jan 12;128(1):101-14.

   Insert

   PICH (ERCC6L Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-FADD

   Plasmid

   #78802

   Purpose

   To overexpress FADD in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

   Insert

   FADD (FADD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A CARD8-FLAG

   Plasmid

   #169948

   Purpose

   Mammalian expression of CARD8-FLAG

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-mTagBFP

    Plasmid

    #75148

    Purpose

    TagBFP (blue) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/BLM

    Plasmid

    #111766

    Purpose

    To express human BLM protein in human cells

    Depositor

    Ian Hickson

    Article

    Gaymes et al Oncogene. 2002 Apr 11;21(16):2525-33. do

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Aiolos in Artichoke

    Plasmid

    #74452

    Purpose

    Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    SFFV and EF1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PLKO.1-UPF1-CDS

    Plasmid

    #136037

    Purpose

    UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_SLC25A5

    Plasmid

    #132007

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A5 (SLC25A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLV-GFP-Sec61β

    Plasmid

    #186597

    Purpose

    Third generation lentiviral vector expressing GFP-Sec61β

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Sec61β (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ462

    Plasmid

    #187970

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV SOCS-3

    Plasmid

    #11486

    Depositor

    Ronald Kahn

    Article

    Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

    Insert

    SOCS-3 (Socs3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-USPL1-pcDNA3.1

    Plasmid

    #85760

    Purpose

    mammalian expression of full length HA tagged USPL1

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge