165,343 results

1. haA1.pBABEpu

   Plasmid

   #21813

   Depositor

   David Ron

   Article

   Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

   Insert

   GADD34 C-term
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. PH-PLCd1-mNGx3

   Plasmid

   #202753

   Purpose

   expresses fluorescent protein-tagged lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-GenEPi

   Plasmid

   #140236

   Purpose

   Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

   Depositor

   Periklis Pantazis

   Article

   Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

   Insert

   GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
   

   Tags

   GCaMP 6s RS1 EF4

   Expression

   Mammalian

   Promoter

   simian CMV IE94

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBR-Cas9ΔyqhD-HA500

   Plasmid

   #220369

   Purpose

   A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

   Depositor

   Yilan Liu

   Article

   Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

   Inserts

   SpCas9
   sgRNA for yqhD in P. putida KT2440
   

   Use

   CRISPR; Broad host range

   Expression

   Bacterial

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-EGFP-RhoA-Q63L

   Plasmid

   #12968

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA constitutively active (RHOA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q63L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXG237

   Plasmid

   #141281

   Purpose

   ATF4 translational reporter

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   uORF1/2 of ATF4 followed by mApple
   

   Use

   Lentiviral

   Tags

   mApple

   Promoter

   CMV

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

   Plasmid

   #223747

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Lambda-PPase
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. SepRS(2)/pSertRNA(B4)/EF-Sep

   Plasmid

   #173897

   Purpose

   The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

   Depositor

   Jason W Chin

   Article

   Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

   Inserts

   SepRS(2)
   pSertRNA
   EF-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mScarlet-EEA1

   Plasmid

   #169067

   Purpose

   Express human EEA1 in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   EEA1 (EEA1 Human)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBad-LgBiT-PhoCl1-SmBiT-MBP

    Plasmid

    #164034

    Purpose

    luciferase-based PhoCl screening construct

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    LgBiT-PhoCl1-SmBiT-MBP
    

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Promoter

    araBad promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_Gal4UAS_IRES_mC_pGK_tBFP

    Plasmid

    #79123

    Purpose

    Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) CircRNA Mini Vector

    Plasmid

    #60648

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSETB/mStayGold(c4)

    Plasmid

    #212018

    Purpose

    For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold(c4)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-RARE-luciferase

    Plasmid

    #13458

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Retinoic Acid Receptor Response Element
    

    Use

    Luciferase

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-GFP-Gal8

    Plasmid

    #127191

    Purpose

    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Insert

    GFP-Gal8 (LGALS8 Human)
    

    Tags

    Gal8 is fused to the c-terminus of GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-GFP

    Plasmid

    #32395

    Depositor

    John T Gray

    Article

    Gray et al Methods Mol Biol. 2011;807:25-46.

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_SFFV

    Plasmid

    #79121

    Purpose

    Lentiviral vector for constitutive expression of genes (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCVneo-HA-ER-Hoxb8

    Plasmid

    #222291

    Purpose

    The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

    Depositor

    Mark Kamps, David Sykes

    Article

    Wang et al Nat Methods. 2006 Apr;3(4):287-93.

    Insert

    ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The estrogen receptor hormone binding domain cont…

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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