4,168 results

1. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBB540

   Plasmid

   #27393

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   grpE, clpB
   

   Expression

   Bacterial

   Available Since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCE-mp53DD

   Plasmid

   #41856

   Purpose

   Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   Trp53 (Trp53 Mouse)
   

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   CAG

   Available Since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. DLX2-hygro

   Plasmid

   #97330

   Purpose

   Generating lentiviruses to express Dlx2 and hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   DLX2 (Dlx2 Mouse)
   

   Use

   Lentiviral

   Promoter

   TetO

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBB542

   Plasmid

   #27395

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   dnaK, dnaJ, groESL
   

   Expression

   Bacterial

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-Ascl1-puro

   Plasmid

   #97329

   Purpose

   Generating lentiviruses to express Ascl1 and puromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Promoter

   TET-ON

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mNeonGreen-Giantin

   Plasmid

   #98880

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Giantin
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a-Traptavidin

   Plasmid

   #24753

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

   Insert

   Traptavidin
   

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G an…

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT4/HB

    Plasmid

    #108352

    Purpose

    Optimized Sleeping Beauty Transposon Vector with MCS

    Depositor

    Wolfgang Uckert

    Article

    Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

    Type

    Empty backbone

    Use

    Transposon

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Synthetic, Human)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-HDAC4

    Plasmid

    #45636

    Depositor

    Tso-Pang Yao

    Article

    Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2 neo

    Plasmid

    #98292

    Purpose

    Variant of lentiCRISPRv2 that confers G418 resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. ClopHensorN

    Plasmid

    #50758

    Purpose

    Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

    Depositor

    Colin Akerman

    Article

    Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

    Insert

    ClopHensorN
    

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    CAG

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-mt-mKeima

    Plasmid

    #131626

    Purpose

    lentiviral expression of mitochondrial mKeima for generation of stable cell lines

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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