We narrowed to 13,711 results for: cas9

1. pLKO5d.EFS.SpCas9.P2A.BSD

   Plasmid

   #57821

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC1.2/EC1.1_spCas9_GFP

   Plasmid

   #161933

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. M3-M14-dCas9

   Plasmid

   #134779

   Purpose

   Expression of fusion protein M3-M14-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 S. pyogenes, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH47742:FCP:Cas9YFP

   Plasmid

   #85986

   Purpose

   Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR; Golden gate assembly

   Tags

   SV40 NLS, YFP

   Expression

   Mutation

   Promoter

   FCP

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. HBT-pcoCas9

   Plasmid

   #52254

   Purpose

   transient expression of pcoCas9 gene in plant cells

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   pcoCas9
   

   Use

   CRISPR; Plant expression

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   hybrid constitutive promoter 35SPPDK

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-J23109

   Plasmid

   #113151

   Purpose

   Plasmid constitutively expressing dCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A & H840A

   Promoter

   Available since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA-SpCas9-g3

   Plasmid

   #80786

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScI_dCas9-CDA-UL

   Plasmid

   #108551

   Purpose

   Bacterial Target-AID UGI-LVA vector

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Insert

   dCas9-PmCDA1-UGI-LVA
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   lambda OR

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3cH_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104912

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9-sgRNA-A+B

    Plasmid

    #149370

    Purpose

    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, PGK, and U6

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJWV102-PL-dCas9

    Plasmid

    #85588

    Purpose

    Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    dCas9sp
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLsp

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_Anc689-NG-nCas9

    Plasmid

    #163527

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-nCas9-PBE

    Plasmid

    #98160

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Maize

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9-HE-Geminin

    Plasmid

    #109402

    Purpose

    Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    HE-Geminin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Use

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-HypaCas9

    Plasmid

    #190604

    Purpose

    PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-spCas9-SB

    Plasmid

    #169919

    Purpose

    Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.

    Depositor

    Zhili Rong

    Article

    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

    Insert

    SpCas9-SB100X
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9-2A-Cre

    Plasmid

    #68468

    Purpose

    co-expressing Cas9 and Cre recombinase

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    Cas9 and Cre recombinase
    

    Use

    CRISPR

    Tags

    P2A-Cre-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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