Addgene

We narrowed to 1,044 results for: proc.2

Showing: 481 - 500 of 1044 results

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pFCIV-CEACAM6

Plasmid

#149303

Purpose

lentiviral packaging vector for CEACAM6 expression.

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ubiquitin C

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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HDAC3 Flag

Plasmid

#13819

Purpose

Mammalian expression of human histone deacetylase 3 with flag tag

Depositor

Eric Verdin

Article

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC3 (HDAC3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-hTERT

Plasmid

#1773

Purpose

Retroviral expression of hTERT. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-rM3D(Gs)

Plasmid

#45549

Purpose

Expresses rM3D (Gs) neuronal activator

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

rM3D (Gs)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-hTERT-HA

Plasmid

#1772

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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G-GECO1-Orai3

Plasmid

#73563

Purpose

Green fluorescent reporter of Orai3-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1-Orai3 (ORAI3 Human, Synthetic)

Use

Tags

G-GECO1

Expression

Mammalian

Mutation

Promoter

CMV Immdeiate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Use

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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myc-GABAAR Gamma2

Plasmid

#127619

Purpose

N-terminal myc-tagged Gamma2 subunit of GABAAR

Depositor

Josef Kittler

Article

Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

Insert

GABA-A receptor subunit gamma-2

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-hDicer

Plasmid

#89144

Purpose

Expression of hDicer in Sf9 Cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Use

Tags

His6-tev

Expression

Insect

Mutation

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1_WT_OCT4

Plasmid

#40633

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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MELOPS

Plasmid

#80151

Purpose

Genetically encoded melanosome-localized pH sensor.

Depositor

Santiago Di Pietro

Article

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Insert

OCA2 (OCA2 Human)

Use

Tags

mNectarine in first luminal loop

Expression

Mammalian

Mutation

nonpathologic polymorphism D257A, pore mutation V…

Promoter

CMV

Available since

Sept. 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits
HAI

Plasmid

#71316

Purpose

Inducible expression of human arginase I in E. coli

Depositor

David Christianson

Article

DiCostanzo et al Proc Natl Acad Sci U S A. 2005 Sep 13;10

Insert

human arginase I (ARG1 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pCEP4_WT_OCT4

Plasmid

#40629

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
MBP-CRY1-PHR

Plasmid

#26035

Depositor

Johann Deisenhofer

Article

Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

CRY1 (CRY1 Mustard Weed)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pEYFP-Synectin

Plasmid

#35791

Depositor

Arie Horowitz

Article

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Insert

Synectin (Gipc1 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-6p-hDcp2

Plasmid

#72216

Purpose

bacterial expression of GST-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

F146Y and E198K (please see depositor's comm…

Promoter

tac

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMax-Synectin

Plasmid

#35943

Depositor

Arie Horowitz

Article

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Insert

Synectin (Gipc1 Mouse)

Use

Tags

6xHIS and Xpress

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-hDcp2 E147Q, E148Q

Plasmid

#72215

Purpose

bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

E147Q, E148Q

Promoter

T7

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282-LUC

Plasmid

#46865

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

24p3 minimal promoter

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only