177,604 results
-
Plasmid#11344DepositorInsertgateway cassette
UseRNAiExpressionWormAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21b-Casp1-His
Plasmid#11809DepositorAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
evoBxb1
Plasmid#222338PurposePlasmid for expressing evolved Bxb1 in the mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCodon-optimized evolved Bxb1
ExpressionMammalianPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6a
Plasmid#207851PurposeMammalian expression of PE6a prime editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE6a
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationEc48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.3_SARS2_omicron BA.2
Plasmid#183700Purposeexpressing SARS-CoV-2 BA.2 spike protein for pseudovirus productionDepositorInsertSpike (BA.2) (S SARS-CoV-2)
UseLentiviral and RetroviralExpressionMammalianMutationc-terminal 18 aa deletionPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hLRP1-pEZT-BM
Plasmid#177985PurposeExpression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLDL receptor related protein 1 (LRP1 Human)
TagsNoExpressionMammalianPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-EF1a-CreERT2-3Xflag-T2A-eBFP2
Plasmid#170186PurposeThis Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracingDepositorInsertCreERT2-T2A-eBFP2
UseLentiviralPromoterEF1 alphaAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…MutationF70A/F108YAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19 - T7 pro - IRES - EGFP
Plasmid#138586PurposeExpress EGFP with an upstream T7 promoter in pUC19 backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLifeAct-HyPer7
Plasmid#136464PurposeMammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHyPer7
TagsLifeActExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-APOBEC1-YTHmut
Plasmid#131637PurposeExpresses APOBEC1 fused to a mutant YTH domain with diminished m6A bindingDepositorInsertAPOBEC1-YTHmut-HA (YTHDF2 Human, Rat)
TagsHAExpressionMammalianMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBT375
Plasmid#125616PurposeExpresses evoAPOBEC1-BE4max-NG in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertevoAPOBEC1-BE4max-NG
ExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
Plasmid#118154PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationSee depositor comments belowPromoterEF1a core and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianPromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
Tags6x-HisExpressionBacterialPromoterT7 promoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (mCherry)
Plasmid#102945PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)DepositorInsertSIINFEKL (OVAL Synthetic, Chicken)
UseRetroviralExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-GACh2.0
Plasmid#106073Purposeexpress the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based ACh sensor (GACh) 2.0
ExpressionMammalianPromoterCMVAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXPR_047
Plasmid#107145Purposemeasures Cas9 activityDepositorInsertGFP + sgRNA for GFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSyn.eGFP.WPRE.bGH
Plasmid#105539PurposeAAV expression of EGFP from hSyn promoterDepositorHas serviceAAV1InsertEGFP
UseAAVExpressionMammalianPromoterhSynAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by