We narrowed to 17,934 results for: multi
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Plasmid#31396DepositorInsertpJT122
UseSynthetic BiologyExpressionBacterialAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
ExpressionMammalianMutationA215CPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHB-3
Plasmid#140956PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRE-iRFP670
Plasmid#82696PurposeExpresses CRE and iRFP670 under two separate PGK promoters.DepositorInsertCRE, iRFP670
UseCre/LoxAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA
Plasmid#171619PurposeAAV floxed Soma-targeted Cal-Light with KA2DepositorInsertIE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…ExpressionMammalianPromoterCMVAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorExpressionBacterialPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
Plasmid#26797PurposeHuman 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately.DepositorInsertsTagsmCherry-P2AExpressionMammalianMutationD100APromoterEF1α and short TetO, miniCMVAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…PromoterxylR-driven promoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-pGK:GFP
Plasmid#68486PurposeRetrovirus expressing GFP driven by pGK promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralExpressionMammalianPromoterLTR and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF_dCas9-VP64
Plasmid#68417PurposeTransient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAG and VP64ExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-myrSNAP-CON
Plasmid#60099PurposeExpresses membrane-targeted SNAP(f)-tag and histone-GFP, conditionalDepositorInsertsSNAP-tag
histone-GFP
UseAAV and Mouse TargetingExpressionMammalianAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINEneo-3C-GFP
Plasmid#53534PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
TagsGFP-His8ExpressionBacterial, Insect, and Mamm…Available sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSLGFP
Plasmid#51494PurposepLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmidDepositorInsertsGFP
us:NPTII repair template
UsePlant t-dna plasmidTagsNLS and flagPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAM2195
Plasmid#40245DepositorPublicationInsertPpsbAI::luxCDE, PpsbAI::luxAB
UseCyanobacteria cloning vectorPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC158-3XUAS-IVS-B3::PEST
Plasmid#32138DepositorPublicationInsertB3::PEST
ExpressionInsectAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTU424
Plasmid#178983PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)DepositorInsertTPR-CHAT
TagsHis-tagExpressionBacterialPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBR_Cas9_sp1
Plasmid#154851PurposeExpresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genomeDepositorInsertsSpcas9 gene
sgRNA scaffold
UseCRISPRTags6xHisMutationCodon harmonized variant for expression in Rhodob…Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only