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176,036 results

Showing: 5041 - 5060 of 176036 results

  11. mCherry-NbALFA

    Plasmid
    #159987
    Purpose
    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Publication
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mCherry-NbALFA
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mCherry and NbALFA
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159987-plasmid-map-sequence-id-314745

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  12. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    139988-plasmid-map-sequence-id-275338

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  14. pX458M-53BP1-DN1S

    Plasmid
    #131045
    Purpose
    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.
    Depositor
    Punam Malik
    Publication
    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:
    Insert
    SpCas9-53BP1-DN1S (TP53BP1 Human)
    Use
    CRISPR
    Tags
    GFP and SpCas9
    Expression
    Mammalian
    Mutation
    Only expressing residues 1231-1644 of complete 53…
    Promoter
    CBH
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    131045-plasmid-map-sequence-id-258265

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  16. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    118154-plasmid-map-sequence-id-239465

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Showing: 5041 - 5060 of 176036 results