162,160 results

1. SP6-VEE-IRES-Puro

   Plasmid

   #58971

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ404-DogCatcher-sfGFP

   Plasmid

   #171930

   Purpose

   Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher-sfGFP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. HRE-dUnOHR

   Plasmid

   #201714

   Purpose

   Hypoxia responsive promoter driving UnaG and mOrange

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV HA hRB-wt

   Plasmid

   #58905

   Purpose

   mammalian expression of wild type human Rb

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. rGluA2 (px330:2-guideCas9)

   Plasmid

   #169439

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCK122 pDESTattB_exorh:mCherry

   Plasmid

   #213511

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYLCRISPR/Cas9Pubi-H

   Plasmid

   #66187

   Purpose

   expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   maize ubiquitin promoter (Pubi)

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC1-human APPL1

   Plasmid

   #22198

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-mNaChBac-T2A-tdTomato

   Plasmid

   #60650

   Purpose

   Expresses mNaChBac-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   mNaChBac-T2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Non tagged Avidin

    Plasmid

    #214835

    Purpose

    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Non tagged Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PT2/C-Luc//PGK-SB13

    Plasmid

    #20207

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    Flirefly luciferase/Sb transposase
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid

    #104563

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1 (D832A)
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Sport6-CD63-pHuji

    Plasmid

    #130902

    Purpose

    Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHuji (CD63 Human, synthetic)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    pHuji inserted between Gln-36 and Leu-37 in extra…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHes1-DN-GFP (CC#110)

    Plasmid

    #15134

    Depositor

    Connie Cepko

    Article

    Furukawa et al Neuron. 2000 May . 26(2):383-94.

    Insert

    Hes1 (Hes1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    E43A, K44A, R47A

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1_GEM4-Halo-FRB

    Plasmid

    #197057

    Purpose

    Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_GEM7-Halo-FRB

    Plasmid

    #197058

    Purpose

    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18T-mini-Tn7T-hph-Ptet1

    Plasmid

    #218367

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.Syn.GCaMP6f.WPRE.SV40

    Plasmid

    #100837

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid

    #108542

    Purpose

    Lentivirus vector expressing Luciferase with blasticidin resistance

    Depositor

    Javier Alcudia

    Inserts

    Luciferase
    Neomycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1apha and PGK

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-SNAP-PI(3,4,5)P3 Probe

    Plasmid

    #211514

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BTK1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUCmini-iCAP-AAV1.X1

    Plasmid

    #209780

    Purpose

    non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV1-X1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-PIPK1 gamma 90

    Plasmid

    #22299

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBS-Int

    Plasmid

    #50000

    Purpose

    Expresses the integrase on a suicide plasmid

    Depositor

    Brian Robertson, Peter Sander

    Article

    Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

    Insert

    Integrase from pMV361
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    own

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pMS2M-PH-gagpol-D64V

    Plasmid

    #166031

    Purpose

    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Insert

    MS2-Gag-Pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D64V mutation within the integrase within Pol

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHes1-DsRed

    Plasmid

    #13767

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Hes1 promoter
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Frt-V5-hspB1

    Plasmid

    #63102

    Purpose

    Expression of HSPB1 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLPCX2-MT1-MMP-eGFP

    Plasmid

    #89819

    Purpose

    Express in MT1-MMP mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    MT1-MMP (MMP14 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p11L1w

    Plasmid

    #37330

    Depositor

    John Schiller

    Article

    Mossadegh et al Virology. 2004 Aug 15;326(1):57-66.

    Insert

    HPV11 L1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge