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Addgene

We narrowed to 5,415 results for: PEP;

Showing: 5101 - 5120 of 5415 results
  1. EBL1-bio

    Plasmid
    #47792
    Purpose
    Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Insert
    Codon-optimised EBL1
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. Cntn3-AP-His

    Plasmid
    #71941
    Purpose
    Expresses the extracellular region of the Contactin 3 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Insert
    Cntn3 (Cntn3 Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3-bio-myc-mCE 2-210

    Plasmid
    #112715
    Purpose
    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells
    Depositor
    Insert
    Rngtt (Rngtt Mouse)
    Use
    Tags
    biotinylation signal peptide (Tagwerker et al. 20…
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. Cntn3-Fc-His

    Plasmid
    #72067
    Purpose
    Expresses the extracellular region of the Contactin 3 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Insert
    Cntn3 (Cntn3 Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. TLP-bio

    Plasmid
    #47787
    Purpose
    Expresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Insert
    Codon-optimised TLP
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. PF3D7_1431400-bio

    Plasmid
    #47791
    Purpose
    Expresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Insert
    Codon-optimised PF3D7_1431400
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid
    #233064
    Purpose
    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.
    Depositor
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid
    #233063
    Purpose
    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Threonine 923 to Glycine
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 5101 - 5120 of 5415 results