164,826 results

1. pcDNA3 HA Arf1 DN T31N

   Plasmid

   #10833

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf1 T31N (ARF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T31N. Defective in GTP binding. Dominant negati…

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-Halotag-FRB-EX-nes_pLX304

   Plasmid

   #120913

   Purpose

   Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   HA-Halotag-FRB-EX-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti.PGK.chFP.W

   Plasmid

   #51008

   Purpose

   Lentivector that expresses chFP from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   mChFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-D50 lamin A

   Plasmid

   #17653

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   D50 lamin A (LMNA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50 (50 aa internal deletion in globular tai…

   Promoter

   CMV

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-Frb-v1

   Plasmid

   #201923

   Purpose

   E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   FrbA
   FrbB
   FrbC
   FrbD
   FrbE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (modified), T7 (modified, moderate), T7 (modif…

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PGK-Hygro-PIP-FUCCI

   Plasmid

   #138714

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p11-LacY-wtx1

   Plasmid

   #69056

   Purpose

   The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

   Depositor

   Huimin Zhao

   Article

   Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

   Inserts

   ccdB (ccdB )
   lacY (lacY )
   

   Use

   Tags

   A177C

   Expression

   Mutation

   Promoter

   pBAD and pLac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-HA-USP36

   Plasmid

   #22579

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP36 (USP36 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-S5E2-dTom-nlsdTom

    Plasmid

    #135630

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGLO-Blue

    Plasmid

    #220782

    Purpose

    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    blue chromoprotein (aeBlue)
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXSE901BG

    Plasmid

    #91714

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK317

    Plasmid

    #59571

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid

    #219828

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3-ApaLID211A-HA

    Plasmid

    #211459

    Purpose

    Express mitochondrial localized ApaLI with D211A mutation

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-D211A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D211A

    Promoter

    CMV

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBZS-YP_009702000

    Plasmid

    #198379

    Purpose

    Express YP_009702000 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_009702000 (CVA-1_913R )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ClopHensorN

    Plasmid

    #50758

    Purpose

    Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

    Depositor

    Colin Akerman

    Article

    Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

    Insert

    ClopHensorN
    

    Use

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    CAG

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FlipCherry-T2A-GFP

    Plasmid

    #124436

    Purpose

    Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipCherry-T2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid

    #208717

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Has Service

    AAV1, AAV2, and AAV8

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1-HA-NONO

    Plasmid

    #127655

    Purpose

    Over-express HA-tagged human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNeae2

    Plasmid

    #168300

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

    Insert

    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    

    Use

    Tags

    6xHis, E-tag, and myc-tag

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSCAR_sgRNA_puro-mKate-lox2272

    Plasmid

    #162076

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid

    #113950

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. TFORF3298

    Plasmid

    #144774

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmStayGold_mStayGold_I3

    Plasmid

    #239339

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mARGAna (cassette 1)

    Plasmid

    #191341

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. paavCAG-pre-mGRASP-mCerulean

    Plasmid

    #34910

    Purpose

    pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMXSIP-FLAG-ATPIF1

    Plasmid

    #204703

    Purpose

    expresses FLAG-ATPIF1 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge