162,160 results

1. mCherry-ZO1-C-14

   Plasmid

   #55165

   Purpose

   Localization: Tight Junctions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   ZO1 (TJP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLminP_Luc2P_RE53

   Plasmid

   #90398

   Purpose

   STAT1 - STAT1 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   STAT1

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBPGUw

   Plasmid

   #17575

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Tools for neuroanatomy and neurogenetics

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-Vinculin-23

   Plasmid

   #55159

   Purpose

   Localization: Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-tMCK-ER-TurboID

   Plasmid

   #149412

   Purpose

   Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   ER-TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mutation

   Promoter

   tMCK

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-ATR WT

   Plasmid

   #31611

   Depositor

   Aziz Sancar

   Article

   Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

   Plasmid

   #187588

   Purpose

   Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NLS
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDSP33

   Plasmid

   #199375

   Purpose

   Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Promoter

   Available since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pBJ FLAG-hPar1

   Plasmid

   #53226

   Purpose

   mammalian expression of human Par1 with N-terminal FLAG tag

   Depositor

   Shaun Coughlin

   Article

   Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

   Insert

   Par1 (F2R Human)
   

   Use

   Tags

   FLAG and bovine prolactin signal sequence (MDSKGS…

   Expression

   Mammalian

   Mutation

   Deletion of aa1-39 (native signal peptide)

   Promoter

   SV40

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mTurquoise2-N1

    Plasmid

    #54843

    Purpose

    Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

    Depositor

    Dorus Gadella, Michael Davidson

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. CMV-hRad51

    Plasmid

    #125570

    Purpose

    Expresses the Rad51 protein in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51 (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MMLVgag-3NES-Cre

    Plasmid

    #207252

    Purpose

    Generation of CreVLPs when used in conjunction with MMLV gag-pol during production

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    MMLVgag-3NES-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAIP hIL4 co

    Plasmid

    #74169

    Purpose

    Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL4 (IL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Rab5C-S35N

    Plasmid

    #110503

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou

    Insert

    Rab5C-S35N (RAB5C Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S35N

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIVM_26

    Plasmid

    #204501

    Purpose

    Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    0

    Promoter

    AmpR

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-3xFLAG-V5-ccdB

    Plasmid

    #87064

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFPC2-CB2 SH3+

    Plasmid

    #68821

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CXCR2-DuET

    Plasmid

    #213219

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR2 (CXCR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAK_DR30_CasRx_mCherry

    Plasmid

    #134844

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL2-Full

    Plasmid

    #16012

    Depositor

    Wafik El-Deiry

    Article

    Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

    Insert

    KILLER/DR5 (TNFRSF10B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74010

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5xUAS
    Cas9
    Cre
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100852

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHS15 (hANGPTL3-Strep)

    Plasmid

    #109112

    Purpose

    Expresses StrepII-tagged human ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (ANGPTL3 Human)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ER-mRFP

    Plasmid

    #62236

    Purpose

    Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.

    Depositor

    Erik Snapp

    Article

    Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;10

    Insert

    ER-mRFP
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197064

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mScarlet-Rab9a

    Plasmid

    #169074

    Purpose

    Express human Rab9a in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab9a (RAB9A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSLTS

    Plasmid

    #59386

    Purpose

    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Type

    Empty backbone

    Use

    Genome engineering

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CMV-dSa-VPR mini.-syn pA

    Plasmid

    #99689

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge