We narrowed to 23,191 results for: his
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Plasmid#26049DepositorAvailable SinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only
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pGP-CMV-jGCaMP8f F366H
Plasmid#204141PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutationDepositorInsertjGCaMP8f F366H
TagsN-terminal His tagExpressionMammalianMutationPhenylalanine 366 changed to histidinePromoterCMVAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124A wgMDH
Plasmid#204257PurposeBacterial expression of R124A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124AAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD11
Plasmid#31333DepositorInsertproteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDYH5
Plasmid#52032PurposeExpression of GFP-tagged DAF-16 in C. elegansDepositorAvailable SinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-ZF5.3-GFP-HiBit
Plasmid#170992PurposeFor bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b SARS-CoV PLpro
Plasmid#61693Purposeinducible expression of SARS PLpro proteaseDepositorInsertSARS-CoV PLpro
TagsHisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 MEKK1 KD
Plasmid#12180DepositorInsertMEKK1 (Map3k1 Mouse)
TagsHis and XpressExpressionMammalianMutationKinase dead: K1253M; V1467LAvailable SinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV gfaABC1D NES-jRCaMP1a
Plasmid#171120PurposeAstrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.DepositorInsertjRCaMP1a
UseAAVTagsHis Tag, T7 Tag, XpressTagExpressionMammalianPromotergfaABC1DAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mAmetrine-CNA35
Plasmid#61604Purposebacterial expression of N-terminal fusion protein of CNA35 with mAmetrineDepositorInsertmAmetrine and CNA35
TagsHis-tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF2
Plasmid#228776PurposeProtein expression plasmid for YTH domain of YTHDF2 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MPP6
Plasmid#34806DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
Tags6 his tagExpressionBacterialAvailable SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-hTR
Plasmid#21811DepositorAvailable SinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL
Plasmid#169105PurposeExpresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mfn2(K109A)-16xmyc
Plasmid#26051DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80Q
Plasmid#118351PurposePlasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-POC5
Plasmid#136786PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tagDepositorInsertSNAP-POC5 (POC5 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6A-EGFR ICD (645-1186)
Plasmid#42667DepositorAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-TFGbeta receptor II K277R
Plasmid#11762DepositorInsertTGFbeta receptor II (TGFBR2 Human)
TagsHisExpressionMammalianMutationK277R: kinase deadPromoterCMVAvailable SinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)
Plasmid#164184PurposeFreeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianPromoterSynAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-DDX4LCD
Plasmid#204408PurposeE. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-STIL
Plasmid#136795PurposeMammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tagDepositorInsertSNAP-STIL (STIL Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
TagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
TagsHis-SUMOExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
BtuF
Plasmid#92209PurposeExpression of BtuF which is the vitamin B12 binding protein for BtuCD ABC TransporterDepositorInsertBtuF (btuF )
Tags6x His Tag and PelB Signal SequenceExpressionBacterialMutationBtuF signal sequence removed and uses the PelB si…PromoterT7 PromoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectPromoterpolyhedrinAvailable SinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STMN1
Plasmid#31326DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertExpressionBacterialAvailable SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET24a-AlkB deltaN11
Plasmid#73622PurposeExpresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tagDepositorInsertAlkB (alkB E. coli)
TagsHisExpressionBacterialMutationdeletion of first 11 AAPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-PREI3
Plasmid#31592DepositorAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only