We narrowed to 22,195 results for: his

1. D20_Ld_140560

   Plasmid

   #135836

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, unknown function
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. D16_Ld_090240

   Plasmid

   #135832

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. D15_Ld_061240

   Plasmid

   #135831

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   thioredoxin, putative
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. D13_Ld_050900

   Plasmid

   #135829

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   surface antigen- like protein
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. D12_Ld_040370

   Plasmid

   #135828

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. D11_Ld_211610

   Plasmid

   #135827

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. D7_Ld_363330

   Plasmid

   #135823

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   receptor-type adenylate cyclase a-like protein
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. D6_Ld_100150

   Plasmid

   #135822

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   nucleoside phosphatase, putative
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. D65_Ld_051210

   Plasmid

   #135878

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   surface antigen-like protein
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. D3_Ld_030930

    Plasmid

    #135819

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    multicopper oxidase, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. D2_Ld_030320

    Plasmid

    #135818

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    DDRGK domain containing protein, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. D1_Ld_290860

    Plasmid

    #135817

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    cysteine peptidase C (CPC)
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. D14_Ld_051070

    Plasmid

    #135830

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    Legume-like lectin family, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. D10_Ld_292250

    Plasmid

    #135826

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    C-8 sterol isomerase-like protein
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. D17_Ld_090870

    Plasmid

    #135833

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, conserved
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. D8_Ld_340400

    Plasmid

    #135824

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, unknown function
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDK616

    Plasmid

    #134202

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K223A K226A

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJS263

    Plasmid

    #134141

    Purpose

    Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    Ska1 MTBD-His (Y106G6H.15 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIW087

    Plasmid

    #134128

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1 ; human Ska2
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    K183A K184A

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIW090

    Plasmid

    #134129

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1 ; human Ska2
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    K223A K226A

    Promoter

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28b-FOXP2_P1

    Plasmid

    #122645

    Purpose

    Expresses truncated variant (aa271-aa714) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28b-FOXP2_P3

    Plasmid

    #122647

    Purpose

    Expresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28b-FOXP2_P4

    Plasmid

    #122648

    Purpose

    Expresses truncated variant (aa503-aa714) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVITRO-Fab_Theo-LUCID

    Plasmid

    #119048

    Purpose

    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVITRO-Fab_Quin-LUCID

    Plasmid

    #119049

    Purpose

    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVITRO-Fab_MTX-LUCID

    Plasmid

    #119047

    Purpose

    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-Hook3 Q147A, M151A, I154A

    Plasmid

    #74616

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Q147A, M151A, I154A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-Hook3 D102A, E108A

    Plasmid

    #74618

    Purpose

    Expresses a double mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-Hook3 N68A, W69A, K77A

    Plasmid

    #74619

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCellFree_G03 STAT1

    Plasmid

    #67116

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pCellFree_G03 GTF2A1L

    Plasmid

    #67096

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1L (GTF2A1L Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pCellFree_G03 AKT1

    Plasmid

    #67102

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pCellFree_G03 GTF2H1

    Plasmid

    #67108

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H1 (GTF2H1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pCellFree_G03 TP53

    Plasmid

    #67078

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TP53 (TP53 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pCellFree_G03 E-Cad

    Plasmid

    #67046

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    E-Cad (CDH1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pCellFree_G03 TCEB2

    Plasmid

    #67049

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCEB2 (ELOB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    M6R and Q115K

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. CFP(159-238) in pcDNAI/Amp

    Plasmid

    #60896

    Purpose

    This vector attaches CFP(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    CFP(159-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn-164 to His

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMT mir-1-1 reporter hsa-mir-205

    Plasmid

    #46744

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-205 (MIR205 Human)
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (MT)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-Cb2

    Plasmid

    #53529

    Purpose

    Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

    Depositor

    Gabor Igloi

    Article

    Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

    Insert

    mitochondrial arginyl-tRNA synthetase
    

    Use

    Tags

    His Tag and Thioredoxin

    Expression

    Bacterial

    Mutation

    changed Ser4 to Ala4 to permit NcoI cloning

    Promoter

    T7

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only