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159,352 results

Showing: 5161 - 5180 of 159352 results

  2. TRUPATH Triple Gai3

    Plasmid
    #196050
    Purpose
    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196050-plasmid-map-sequence-id-385864

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  5. HOP-flash

    Plasmid
    #83467
    Purpose
    TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83467-plasmid-map-sequence-id-350804

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  7. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. IFP2.0-H2B-6

    Plasmid
    #56273
    Purpose
    Localization: Nucleus/Histones, Excitation: 684, Emission: 708
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    IFP2.0
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56273-plasmid-map-sequence-id-173183

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  10. p403 – TDH3-MTS-mCherry-GFP1-10-His

    Plasmid
    #177730
    Purpose
    mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    mCherry-GFP1-10
    Use
    Tags
    Mitochondrial Targeting Sequence
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177730-plasmid-map-sequence-id-352610

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  11. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  14. Pet28a_Synthetic_Human_H2A.1

    Plasmid
    #42634
    Depositor
    Joe Landry
    Insert
    Human H2A.1 (H2AC13 Synthetic, Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42634-plasmid-map-sequence-id-57749

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  18. DCIP

    Plasmid
    #193860
    Purpose
    Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.
    Depositor
    Markita Landry
    Article
    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:
    Insert
    NLS-mCherry-GFP1-10
    Use
    Tags
    SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193860-plasmid-map-sequence-id-391139

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Showing: 5161 - 5180 of 159352 results