19,592 results

1. gRNA_AAVS1-T1

   Plasmid

   #41817

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T1
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM2991

   Plasmid

   #40248

   Depositor

   Susan Golden

   Article

   Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

   Insert

   One Step Cloneing Vector for Overexpression
   

   Use

   Cyanobacteria cloning vector

   Promoter

   n/a

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-mhYFP

   Plasmid

   #186533

   Purpose

   Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Monomeric hyperfolder YFP
   

   Expression

   Mammalian

   Mutation

   hfYFP-S147P/L195M/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAL-XPG

   Plasmid

   #39215

   Depositor

   Aziz Sancar

   Article

   Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

   Insert

   XPG (ERCC5 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Note that XPG starts at amino acid 5

   Promoter

   Ptac

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. METTL3 shRNA 2 tet pLKO puro

   Plasmid

   #162984

   Purpose

   Tet-inducible shRNA targeting human METTL3 #2

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Lentiviral

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMK001

   Plasmid

   #33780

   Depositor

   Adam Cohen

   Article

   Kralj et al Science. 2011 Jul 15;333(6040):345-8.

   Insert

   Proteorhodopsin Optical Proton Sensor
   

   Expression

   Bacterial

   Mutation

   D97N

   Promoter

   AraBAD

   Available Since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH47761

   Plasmid

   #48003

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

   Plasmid

   #140220

   Purpose

   Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   dLbCpf1
   

   Use

   CRISPR

   Tags

   miniVPR-mCherry

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   PGK

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SuperNova Green/ pRSETB

   Plasmid

   #124839

   Purpose

   bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

   Insert

   SuperNova Green
   

   Tags

   No tag

   Expression

   Bacterial

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HIP1R-GFP-FKBP

    Plasmid

    #100752

    Purpose

    Expression of HIP1R with a GFP-FKBP tag in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    HIP1R (HIP1R Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Tags

    6His, TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Kif1bβ-pcDNA3

    Plasmid

    #22534

    Depositor

    William Kaelin

    Article

    Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

    Insert

    Kif1bβ (KIF1B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. SuperNova-N1

    Plasmid

    #131853

    Purpose

    Expresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning site

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF.hHES1.Ubc.GFP

    Plasmid

    #17624

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    HES1 (HES1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PL

    Plasmid

    #20783

    Depositor

    Bryan Cullen

    Article

    Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

16. RP1B FUS 1-163

    Plasmid

    #127192

    Purpose

    Protein expression for affinity purification

    Depositor

    Nicolas Fawzi

    Article

    Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

    Insert

    FUS 1-163 (FUS Human)
    

    Tags

    hexahistidine

    Expression

    Bacterial

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRE3G-Bi-gePSI-pCMV-AcGFP

    Plasmid

    #131008

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Promoter

    pCMV and pTRE3G-Bi

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUS_anion

    Plasmid

    #98656

    Purpose

    Expresses untagged FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Expression

    Bacterial

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSIL-eGFP

    Plasmid

    #52675

    Purpose

    shRNA silencing plasmid with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Type

    Empty backbone

    Use

    RNAi

    Tags

    CMV-EGFP

    Promoter

    CMV and U6

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIP-SFFV-mtagBFP-2A STING V155M

    Plasmid

    #102591

    Purpose

    Lentivector to express STING V155M and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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