20,201 results

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pCMW35 p21 (mouse)

Plasmid

#16478

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 (Cdkn1a Mouse)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pcdna3mciap1

Plasmid

#11474

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap1 (Birc2 Mouse)

Expression

Mammalian

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
AAVS1 T2 CRIPR in pX330

Plasmid

#72833

Purpose

CRISPR/Cas to target the AAVS1 locus in human cells

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Cas9 and gRNA for targeting the AAVS1 locus in human cells

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBb(RSF1010)1k-GFPuv

Plasmid

#106395

Purpose

IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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CRISPRoff-v2.1

Plasmid

#167981

Purpose

Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)

Tags

2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

Expression

Mammalian

Promoter

CAG

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro

Plasmid

#21915

Purpose

3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

Depositor

Dmitri Wiederschain

Article

Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLVX-puro-cOVA

Plasmid

#135073

Purpose

Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

Depositor

Maria Castro

Insert

cOVA (OVAL Chicken)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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NbV5-eGFP

Plasmid

#201473

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-eGFP

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-MAFB-IRF8-CEBPA

Plasmid

#190720

Purpose

For microglia differentiation from iPSC

Depositor

Li Gan

Article

Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

Insert

MAFB-IRF8-CEBPA

Expression

Mammalian

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-R-Creon shRNA[Control]

Plasmid

#180775

Purpose

This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV2A

Plasmid

#224440

Purpose

Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDQTTL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro LUC (w168-1)

Plasmid

#17477

Purpose

3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGal3

Plasmid

#73080

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin-3 (LGALS3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1

Plasmid

#116737

Purpose

Lentiviral expression of ESR1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFREE_Zeo

Plasmid

#92053

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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MSCVneo-HA-ER-Hoxb8

Plasmid

#222291

Purpose

The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

10xHis and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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