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Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
UseTagsExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIAA5::LUC
Plasmid#141169PurposeLuminescent reporter for auxin signalingDepositorInsertAtIAA5 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtIAA5, At1g15580Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWKS1583
Plasmid#70188PurposeModular vector for secreted amylase reporter expressionDepositorInsertamyEopt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta4
Plasmid#101292PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation76-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta6
Plasmid#101294PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL189
Plasmid#22278PurposeExpresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitmentDepositorInsertIntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R739A
Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta7
Plasmid#101295PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation727-1354 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-rhodopsin-CTS
Plasmid#86071PurposeA bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.DepositorInsertRhodopsin (RHO Bovine)
UseTagsGSTExpressionBacterialMutationcontains the region from 309-348 of RhodopsinPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAZ10::LUC
Plasmid#141172PurposeLuminescent reporter for jasmonic acid signalingDepositorInsertAtJAZ10 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtJAZ10, AT5G13220Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta5
Plasmid#101293PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation375-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWRKY70::LUC
Plasmid#141171PurposeLuminescent reporter for salicylic acid signalingDepositorInsertAtWRKY70 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtWRKY70, AT3G56400Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Fast-1 H83R
Plasmid#16522DepositorInsertFAST-1 H83R (FOXH1 Human)
UseTagsExpressionMammalianMutationH83R mutantPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2 YFP-Smo-Weyer's
Plasmid#41014DepositorInsertsmoothened homolog (Smo Mouse)
UseTagsEVC2 (aa 1177-1220) and YFPExpressionMammalianMutationYFP inserted after Smo signal sequence. Transfer…PromoterAvailable sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
GST-FAST-FH
Plasmid#16524DepositorInsertFAST-1 (FH domain) (FOXH1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-150. Forkhead domain.PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only