9,006 results

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pEGFP-N1 human cofilin WT

Plasmid

#50859

Purpose

expresses human cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Vinculin-venus

Plasmid

#27300

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Vinculin (VCL Chicken)

Tags

Venus A206K

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCX-cMyc

Plasmid

#19772

Purpose

Non-integrating (episomal) expression of mouse c-Myc

Depositor

Shinya Yamanaka

Article

Okita et al Science. 2008 Nov 7. 322(5903):949-53.

Insert

c-Myc (Myc Mouse)

Expression

Mammalian

Available Since

Jan. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FOXM1c

Plasmid

#68810

Purpose

Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

Depositor

Adam Karpf

Article

Barger et al Oncotarget. 2015 Jul 16.

Insert

FOXM1c (FOXM1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon DREADD Gi-mCherry

Plasmid

#177672

Purpose

Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

Depositor

Karl Deisseroth

Has Service

AAV8

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ152

Plasmid

#69303

Purpose

Gateway entry vector with pco-dCas9-VP64

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-VP64 (Plant codon-optimized)

Use

CRISPR

Mutation

VP64 activator fusion; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Foxo1

Plasmid

#17551

Depositor

Domenico Accili

Article

Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

Insert

pEGFP-N1 Foxo1 (Foxo1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only

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V5-APEX2-LMNA

Plasmid

#129275

Purpose

Expression of APEX2 at nuclear lamina

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-LMNA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47 PINK1 C-GFP

Plasmid

#13316

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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FLAG-MATR3

Plasmid

#32880

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-UBR5

Plasmid

#52050

Purpose

CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

Depositor

Darren Saunders

Article

Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

Insert

UBR5 (UBR5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

Plasmid

#20298

Purpose

Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

channelrhodopsin-2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

humanized ChR2 gene with histidine 134 changed to…

Promoter

EF1alpha

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cre)

Plasmid

#174863

Purpose

Expresses human Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by HsPeg10 UTRs

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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DsRed-rab7 WT

Plasmid

#12661

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector (2A-T)

Plasmid

#29665

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

Plasmid

#162379

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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