8,969 results

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pcDNA3-FLAG-Fos WT

Plasmid

#8966

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-hSOX9-1

Plasmid

#40644

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-hSOX9-1 (SOX9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pQCXI Neo DsRed-LC3-GFP

Plasmid

#31183

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.GCaMP6s.WPRE.SV40

Plasmid

#100845

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-T17N

Plasmid

#22029

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant).

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pJ3H PTP-1B

Plasmid

#8601

Depositor

Ben Neel

Article

Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-

Insert

PTP-1B (PTPN1 Human)

Tags

HA

Expression

Mammalian

Mutation

K103R

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Snail ER

Plasmid

#18798

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

snail (SNAI1 Human)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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Cdk5-DN-HA

Plasmid

#1873

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Tags

HA

Expression

Mammalian

Mutation

D145N, dominant negative

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep164 (Nigg CW324)

Plasmid

#41149

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+ Dkk1-flag

Plasmid

#16690

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Dkk1 (DKK1 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046887

Plasmid

#145730

Purpose

Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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HA-Daxx/pRK5

Plasmid

#52023

Purpose

Expresses HA-Daxx in mammalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

HA-Daxx (DAXX Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP53

Plasmid

#22606

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP53 (USP53 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

not FL, missing first 65 aa

Promoter

CMV/TO

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pME18s-HA-hFIP200

Plasmid

#24303

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

FIP200 (RB1CC1 Human)

Tags

3xHA

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG

Plasmid

#15938

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rubicon

Plasmid

#21636

Depositor

Tamotsu Yoshimori

Article

Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

Insert

Rubicon (1700021K19Rik Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

*(see below)

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT_TO_DESTFLAGHA

Plasmid

#26361

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Type

Empty backbone

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
aMHC-eGFP-Rex-Neo

Plasmid

#21229

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

eGFP

Available Since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP8

Plasmid

#141262

Purpose

Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pARF1(Q71L)-CFP

Plasmid

#11385

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Glutamine 71 to Leucine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pAXEF-Rap1-WT

Plasmid

#32697

Depositor

Vicki Boussiotis

Article

Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

Insert

RAP1 (RAP1A Human)

Expression

Mammalian

Promoter

EF promoter

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 luciferase

Plasmid

#26612

Depositor

Jie Chen

Article

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

firefly luciferase

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
Flag-SENP1m

Plasmid

#17358

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R630L, K631M

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-GATA1-IRES-Puro

Plasmid

#61062

Purpose

lentiviral vector for GATA1

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS424GFP-FYVE(EEA1)

Plasmid

#36096

Depositor

Scott Emr

Article

Burd et al Mol Cell. 1998 Jul;2(1):157-62.

Insert

FYVE(EEA1) domain (EEA1 Human)

Tags

GFP

Expression

Yeast

Promoter

PRC1

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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8,969 results