We narrowed to 17,206 results for: puro

1. pHAGE-3FLAG-TFR1

   Plasmid

   #176490

   Purpose

   For lentiviral transduction of 3FLAG-TFR1

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   TFR1 (TFRC Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRetroX-TRE3G_NINJ1

   Plasmid

   #201181

   Purpose

   Dox-inducible expression of NINJ1 gene in mammalian cells

   Depositor

   Jonathan Kagan

   Article

   Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

   Insert

   Ninjurin 1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSIQmate-EGFP

   Plasmid

   #216241

   Purpose

   All-in One IQ-Cumate transgene switch for the EGFP expression

   Depositor

   Hyunju Ro

   Article

   Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

   Plasmid

   #170578

   Purpose

   Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDKL5 (CDKL5 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   E854G- please see depositor comments

   Promoter

   Ubiquitin

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLPCX2-MT1-MMP-eGFP

   Plasmid

   #89819

   Purpose

   Express in MT1-MMP mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   MT1-MMP (MMP14 Human)
   

   Use

   Retroviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBbi 41BBL-CD86

   Plasmid

   #183244

   Purpose

   Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   CD86, 41BBL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLQ1651-sgRNA(F+E)-sgGal4

   Plasmid

   #100549

   Purpose

   sgGal4-expressing vector modified from Addgene plasmid #51024

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   sgGal4
   

   Use

   CRISPR

   Tags

   sgRNA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCherryGFP-CoV2-WT

   Plasmid

   #177618

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH-SFB-RNF8

   Plasmid

   #99396

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF8

   Depositor

   Michael Huen, Junjie Chen

   Article

   Huen et al Cell. 2007 Nov 30;131(5):901-14. Epub 20

   Insert

   RNF8 (RNF8 Human)
   

   Use

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMRX-IPU-FLAG-NEK9

    Plasmid

    #168269

    Purpose

    Expresses NEK9 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-HA-GPR35

    Plasmid

    #204708

    Purpose

    expresses HA-GPR35 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    G protein-coupled receptor 35 (GPR35 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCW57.1-Luciferase

    Plasmid

    #99283

    Purpose

    All-in-one lentivector with tet-inducible luciferase

    Depositor

    Stephen Tapscott

    Article

    Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid

    #135267

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-TRE3G-LbNOX-Flag

    Plasmid

    #234980

    Purpose

    Expresses LbNOX in mouse cell lines

    Depositor

    Denis Titov

    Article

    Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

    Insert

    LbNOX
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-TRE3G-mitoLbNOX-Flag

    Plasmid

    #234981

    Purpose

    Expresses mitochondrial LbNOX in mouse cell lines

    Depositor

    Denis Titov

    Article

    Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

    Insert

    mito-LbNOX
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSD141B

    Plasmid

    #218139

    Purpose

    Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD56-eGFP-IRES2-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3×Cherry Dam AID Smash

    Plasmid

    #227374

    Purpose

    For inducible expression and cytosolic localization of Dam

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    Dam DNA-adenine methyltransferase
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMRXIP-GFP-PI4KB

    Plasmid

    #221759

    Purpose

    Stable expression of GFP-PI4KB in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PI4KB (PI4KB Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTurquoise2 NES visual barcode

    Plasmid

    #158670

    Purpose

    tagging clonal populations with NES mTurquoise2 fluorescent protein.

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE EF1α dCas9-NED

    Plasmid

    #109369

    Purpose

    Lentiviral vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXPR_206

    Plasmid

    #96920

    Purpose

    for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. V5-LOV-Turbo-NLS

    Plasmid

    #199663

    Purpose

    expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NLS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-opto-hRIP3 dRHIM

    Plasmid

    #208788

    Purpose

    Inducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    RIP3 (RIPK3 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    RHIM domain is removed

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

    Plasmid

    #85715

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p667-UBC-COQ8A-V5-miniTurbo_IDG-K

    Plasmid

    #189885

    Purpose

    Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    COQ8A (COQ8A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid

    #70658

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

28. AICSDP-63: ACTN2-mEGFP

    Plasmid

    #124607

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MCP-ADAR2-DD(E488Q)

    Plasmid

    #170125

    Purpose

    Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD(E488Q)-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTRIPZ-EGFP:Ajuba

    Plasmid

    #108229

    Purpose

    Lentiviral vector for Tet-inducible Ajuba expression

    Depositor

    Kenneth Irvine

    Article

    Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

    Insert

    AJUBA (AJUBA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tet/ON Minimal CMV promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMRX-IPU-mTagBFP2-Sec61B

    Plasmid

    #228136

    Purpose

    Expresses mTagBFP2-Sec61B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Sec61B (SEC61B Human)
    

    Use

    Retroviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs-IP-PEX3-EGFP

    Plasmid

    #228142

    Purpose

    Expresses PEX3-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti_CMV:Flag-Antares

    Plasmid

    #208852

    Purpose

    Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    Antares
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LentiCRISPR v2-sgLKB1 clone2

    Plasmid

    #162126

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHAGE-3FLAG-RAB5A

    Plasmid

    #176488

    Purpose

    For lentiviral transduction of 3FLAG-RAB5A

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVTHM-shN

    Plasmid

    #83046

    Purpose

    Control shRNA pLVTHM-shN

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA Neg Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE-H1

    Available since

    Oct. 24, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pCW57-GFP-2A-Cas9

    Plasmid

    #170805

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

    Depositor

    Morten Frodin

    Article

    Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SadCas9 VPR

    Plasmid

    #188514

    Purpose

    Expresses FLAG tagged SadCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mTAGBFP2 Peroxisome visual barcode

    Plasmid

    #158668

    Purpose

    tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mTAGBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge