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We narrowed to 17,814 results for: multi

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Showing: 5241 - 5260 of 17814 results

  1. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99214-plasmid-map-sequence-id-192369

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  3. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  4. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

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  6. B-GRAM-W

    Plasmid
    #211707
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211707-plasmid-map-sequence-id-419219

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  14. pAC-DELTA

    Plasmid
    #53273
    Purpose
    Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, together with the lcyE gene of Arabidopsis thaliana and thereby produces delta-carotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Promoter
    Trc
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53273-plasmid-map-sequence-id-84075

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Showing: 5241 - 5260 of 17814 results