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We narrowed to 17,934 results for: multi

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Showing: 5241 - 5260 of 17934 results

  3. Tol2-CAG::Mitbow

    Plasmid
    #158994
    Purpose
    Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Publication
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Mitbow
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158994-plasmid-map-sequence-id-311800

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  6. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid
    #110624
    Purpose
    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    LP1B and hU6
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110624-plasmid-map-sequence-id-211314

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  7. pT7-agrBD-I

    Plasmid
    #53439
    Purpose
    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53439-plasmid-map-sequence-id-83233

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  12. M-tdTom-ST1

    Plasmid
    #48678
    Purpose
    Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Insert
    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9
    Use
    CRISPR
    Promoter
    Sp6
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  15. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Publication
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    170185-plasmid-map-sequence-id-333571

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  16. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Publication
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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  17. pcDNA3-Lyn-PlcR

    Plasmid
    #181830
    Purpose
    Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.
    Depositor
    Jin Zhang
    Publication
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Tags
    ECFP, EYFP, and N-terminal targeting sequence fro…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181830-plasmid-map-sequence-id-364215

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  19. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid
    #123362
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.
    Depositor
    Sidi Chen
    Publication
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    Firefly luciferase
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    123362-plasmid-map-sequence-id-240467

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Showing: 5241 - 5260 of 17934 results