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178,697 results

Showing: 7921 - 7950 of 178697 results

  9. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  14. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Publication
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169815-plasmid-map-sequence-id-334563

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  20. LifeAct-mGreenLantern

    Plasmid
    #164459
    Purpose
    Localization of mGreenLantern fluorescent protein to actin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164459-plasmid-map-sequence-id-322382

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  21. pSL1425 (pSPIN, pCDF backbone)

    Plasmid
    #160735
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    160735-plasmid-map-sequence-id-317231

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  22. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159275-plasmid-map-sequence-id-312908

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  23. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid
    #158652
    Purpose
    making transgenics expressing a UAS driven eGFP and NTR 2.0
    Depositor
    Jeff Mumm
    Publication
    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    Use
    Tol2-based transgenic expression in zebrafish/oth…
    Mutation
    F70A/F108Y
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158652-plasmid-map-sequence-id-310824

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  26. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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Showing: 7921 - 7950 of 178697 results