159,352 results
-
Plasmid#127240PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterhSyn1Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorInsertMB21D1 (CGAS Human)
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-pmDH
Plasmid#67897PurposeExpresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cellsDepositorInsertmCherry-pmDH
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mClover3
Plasmid#74236PurposeConstitutive expression of mClover3 in bacteriaDepositorHas ServiceCloning Grade DNAInsertmClover3
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)
Plasmid#189791PurposeExpress wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (HBB Human, Mouse)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV
Plasmid#24828DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp2-mut
Plasmid#192783Purposecontrol probe of G-Flamp2, insensitive to cAMPDepositorInsertG-Flamp2-mut
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro-humanU6-shRNA FASN
Plasmid#82327Purposeused to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)DepositorInsertshRNA to target Fatty Acid Synthase (FASN) (FASN Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9.EF1a-BFP.sgLMNA
Plasmid#98971PurposeCas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.DepositorInsertLMNA sgRNA and spCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-NGN2 (SA Substitutions)
Plasmid#172308PurposeIn vitro transcription of NGN2 (Ser phosphosites substituted by Ala)DepositorInsertNGN2
UseOtherTagsExpressionMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A an…PromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-pCAG-donor(R2Tg)-GFP(intron)
Plasmid#223248PurposeMammalian expression of the R2Tg RNA donorDepositorInsertR2Tg donor
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-Vinculin-N-21
Plasmid#54962PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertVinculin (VCL Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PPP2R1A_WT
Plasmid#81944PurposeGateway Donor vector containing PPP2R1A, part of the Target Accelerator Plasmid Collection.DepositorInsertPPP2R1A (PPP2R1A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T2-Alpha-Actinin-19
Plasmid#58033PurposeLocalization: Alpha Actinin, Excitation: 555, Emission: 584DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR
Plasmid#210523PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA-directcapture
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Bbs4
Plasmid#128868PurposeFor mammalian expression of mouse Bbs4DepositorInsertBbs4 (Bbs4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEXtev-HMG54kd
Plasmid#26030DepositorInsert3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
UseTagsGSTExpressionBacterialMutationCatalytic domain (amino acids 426-488) Methion…PromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
5HT6-YFP-PIPK(D253A)
Plasmid#96811Purposekinase-dead control for 5HT6-YFP-PIPKDepositorInsert5HT6-YFP-PIPK(D253A)
UseTagsYFPExpressionMammalianMutationD253A inactivates kinase activity of PIPKPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-WT-STAT3
Plasmid#71450PurposeRetroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458_KMT2B_2
Plasmid#101075PurposeEncodes gRNA for 3' target of human KMT2BDepositorInsertgRNA against KMT2B (KMT2B Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_KMT2B_1
Plasmid#101074PurposeEncodes gRNA for 3' target of human KMT2BDepositorInsertgRNA against KMT2B (KMT2B Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma2-GFP2
Plasmid#166774PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma2-GFP2 (GNG2 Synthetic, Human)
UseTagsExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Mito-7
Plasmid#58023PurposeLocalization: Mitochondria, Excitation: 555, Emission: 584DepositorInsertMito (COX8A Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)
Plasmid#162877PurposeExpression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALMDepositorInsertpleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
UseTagsmEos2ExpressionMammalianMutationInsert consists of AA1-170PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_Ado1.0
Plasmid#153288PurposeExpress the adenosine sensor GRAB_Ado1.0 in astrocytesDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRL528
Plasmid#58495Purposehelper plasmid for bacterial conjugal DNA transferDepositorInsertencodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
UseTagsExpressionMutationPromoterAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pQC membrane TdTomato IX
Plasmid#37351DepositorInsertmembrane TdTomato
UseTagsPalmitoylation signal*ExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only