We narrowed to 8,446 results for: gnal
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Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterSox10 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CXCR5-DuET
Plasmid#213222PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-SOD2-2A-Catalase-WPRE
Plasmid#67635PurposeAAV vector expressing both SOD2 and mitochondrial targeted CatalaseDepositorUseAAVMutationDeleted the Peroxisome Targeting Signal from Cata…PromoterCMVAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR156-DuET
Plasmid#213267PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR7-DuET
Plasmid#213203PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralMutationD64V mutation within the integrase within PolAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GRM5-DuET
Plasmid#213303PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_haPD-1
Plasmid#226716PurposePlasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)DepositorInsertHigh affinity PD-1 microbody
TagsHA tag and Mating factor alpha secretion signalExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADORA2A-DuET
Plasmid#213174PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-sfGFP
Plasmid#141184PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFPDepositorHas ServiceCloning Grade DNAInsertS surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
TagsSignal peptide from influenza HA and Superfolder …ExpressionMammalianPromoterCMVAvailable SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a1SE
Plasmid#49168PurposepHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
Tagsmyc and pHGFP (pHIuorin)ExpressionMammalianPromoterCMVAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGF-GAS4X
Plasmid#225751PurposeThis plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.DepositorAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 HisMax-V5-GFP-RRBP1
Plasmid#92150PurposeExpresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.DepositorInsertRRBP1 (RRBP1 Human)
TagsCalreticulin signal peptide, EGFP (A207K mutation…ExpressionMammalianMutationIn RRBP1: N951S and T1329L. E1279 is also missing.PromoterCMVAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-GFP
Plasmid#122100PurposeAAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorHas ServiceAAV2InsertGFP
UseAAVTagsNAExpressionMammalianPromoterEF1a(1.1kb short version)Available SinceMay 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CXCR7-DuET
Plasmid#213224PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRB2-DuET
Plasmid#213181PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-ACE2
Plasmid#141185PurposeMammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tagDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-Fc
Plasmid#141183PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-FcDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
TagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and S…ExpressionMammalianPromoterCMVAvailable SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPRC6A-DuET
Plasmid#213299PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TOM20*-SBP-GFP
Plasmid#120173PurposeExpresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)DepositorInsertMitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
TagsSBP and eGFPExpressionMammalianMutationTruncated TOM20: 1-30 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin-R62D
Plasmid#118381PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.DepositorAvailable SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
GABBR1-DuET
Plasmid#213247PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo
Plasmid#234953PurposeHuman codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHLSec2-irisin-his
Plasmid#122729PurposeExpresses in mammalian cells (HEK293):glycosylated and secreted irisin-hisDepositorInsertirisin (ectodomain of FNDC5) (Fndc5 Mouse, Human, Bovine, Rat)
Tagshis and signal sequence for secretionExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
LPAR4-DuET
Plasmid#213331PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GRM1-DuET
Plasmid#213300PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-KIR2DL1(FL)-FLAG
Plasmid#157625PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-KIR2DL4(FL)-FLAG
Plasmid#157624PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)
Plasmid#119826PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP1-bio
Plasmid#47709PurposeExpresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only