We narrowed to 9,085 results for: mel

1. BirA*-HA-NES

   Plasmid

   #214473

   Purpose

   Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   BirA(R118G)
   

   Use

   Lentiviral; Gateway

   Tags

   HA tag and NES (Nuclear Exclusion Sequence)

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   EF-1a

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. XE49 pt Beta-catenin-myc

   Plasmid

   #16840

   Depositor

   Randall Moon

   Article

   Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

   Insert

   pt beta-catenin-myc (ctnnb1.L Frog)
   

   Use

   Xenopus expression

   Tags

   6x myc

   Mutation

   four point mutations introduced into the GSK-3 ph…

   Available Since

   June 25, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pGG184

   Plasmid

   #165604

   Purpose

   Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   'CAGCG' PAM replacing the 'CGGCG&#…

   Promoter

   lac

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-PIWI

   Plasmid

   #11591

   Depositor

   Edward Chan

   Article

   Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

   Insert

   Ago2 (AGO2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain only aa482-869

   Available Since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. BoxB-MS2 adRNA (RAB7A-ACG site)

   Plasmid

   #170155

   Purpose

   AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   BoxB-RAB7A(ACG)-MS2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

   Plasmid

   #63170

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-OLLAS"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MS2-adRNA (5'UAG)

   Plasmid

   #170126

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(5'UAG)-MS2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL1180-HR-PUbEGFP-NLS

   Plasmid

   #176681

   Purpose

   Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   EGFP
   

   Expression

   Insect

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BoxB-MS2 adRNA (RAB7A-UAG site)

   Plasmid

   #170138

   Purpose

   AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   BoxB-RAB7A(UAG)-MS2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-sg-tdTomato90/816

    Plasmid

    #179918

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159672

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    βTub-NLS-hSpCas9-T2A-GFP
    

    Tags

    T2A-GFP

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCETN1-IRFP670

    Plasmid

    #99879

    Purpose

    Expression of CETN1-iRFP670 in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    CETN1 (CETN1 Human)
    

    Tags

    iRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIN-Pdha1

    Plasmid

    #175936

    Purpose

    knockout mouse Pdha1

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Pdha1 gRNA (Pdha1 Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR-Blunt II-TOPO-PTPRD

    Plasmid

    #29705

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Cloning vector

    Mutation

    none

    Available Since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

15. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MCP-ADAR2-DD(E488Q, N496F)

    Plasmid

    #170139

    Purpose

    AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-ADAR2-DD(E488Q, N496F)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p148

    Plasmid

    #62616

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUASTLOTattB_mCherry::Nrv1::vhhGFP4

    Plasmid

    #163926

    Purpose

    Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-R2ACR_142-EYFP

    Plasmid

    #103964

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R2ACR_142
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1161F

    Plasmid

    #183138

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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