We narrowed to 11,999 results for: SHA

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pHIV-INK4A-zsGreen

Plasmid

#110728

Purpose

Mammalian Expression of p16INK4A IRES zsGreen

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p16INK4A IRES zsGreen (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS hygro

Plasmid

#158634

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-2xPH-TAPP1 R211L mutant

Plasmid

#161991

Purpose

Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PH-TAPP1 (PLEKHA1 Human)

Expression

Mammalian

Mutation

R211L

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Crimson

Plasmid

#70683

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

S. pyogenes sgRNA cassette
Crimson Reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Ef1-a and hU6

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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sgGFP_3

Plasmid

#78165

Purpose

sgGFP

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
qTAG-C-miRFP670nano3-Blast-H3C2

Plasmid

#207784

Purpose

Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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CLP1

Plasmid

#155462

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLP1 (CLP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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BRD4-N CRISPR

Plasmid

#140651

Purpose

BRD4 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

BRD4 (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#106435

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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hCGNL1-Myc-HA

Plasmid

#236515

Purpose

For expression of hParacingulinL1 in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGNL1 (CGNL1 Human)

Tags

Myc-HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-BCL6

Plasmid

#139816

Purpose

doxycycline-inducible expression of Human BCL6 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

BCL6 (BCL6 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T190A, E241G, L314M- please see depositor comments

Promoter

TRE-mCMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pg-ODD-EGFP

Plasmid

#87211

Purpose

HIF-1a's ODD domain fused to EGFP

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a ODD domain (HIF1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

ODD domain (AA 530-652)

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-ALX1

Plasmid

#215023

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human ALX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 1 (ALX1 Human)

Use

AAV

Tags

FKBP-V5

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-MAPRE1

Plasmid

#227324

Purpose

Donor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

MAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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DDX21

Plasmid

#155510

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX21 (DDX21 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

T232A

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A_BAF_G47E

Plasmid

#101774

Purpose

Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

G47E mutation

Promoter

EF1a

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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EGFPC2-CB2 SH3-

Plasmid

#68822

Purpose

EGFP tagged Collybistin expression in mammalian cells

Depositor

Shiva Tyagarajan

Article

de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

Insert

Collybistin (Arhgef9 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

Plasmid

#165456

Purpose

Inducible ZEB2 expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

ZEB2 (ZEB2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-CAF1-C-10

Plasmid

#231782

Purpose

Visualization of nucleus in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-CAF1-C-10 (Chaf1a Mouse)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9

Plasmid

#110823

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

Plasmid

#205442

Purpose

production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

Depositor

Jeremy Kay

Article

Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

Insert

Unc5c (Unc5c Mouse)

Use

AAV

Tags

3xHA

Promoter

mini-CMV (with MVM small intron)

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight_A-CREB_PGK-tdTomato

Plasmid

#154264

Purpose

Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

dominant negative CREB

Expression

Mammalian

Promoter

Tight/tetO

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A_BAF

Plasmid

#101773

Purpose

Expresses BAF in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Promoter

EF1a

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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EGFPC2-CB2 SH3+

Plasmid

#68821

Purpose

EGFP tagged Collybistin expression in mammalian cells

Depositor

Shiva Tyagarajan

Article

de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

Insert

Collybistin (Arhgef9 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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WP_010880027.1

Plasmid

#194729

Purpose

Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

Depositor

Fang Li

Article

Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

Insert

nanoparticle-proteinA and His tag

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR2

Plasmid

#218537

Purpose

human FLVCR2 cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR2 (FLVCR2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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