We narrowed to 33,418 results for: IND

1. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

   Plasmid

   #196856

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
   

   Expression

   Mammalian

   Mutation

   Lacking the centrosome-targeting domain (CTD)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

   Plasmid

   #170202

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

   Plasmid

   #170203

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

   Plasmid

   #170191

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417N, E484K, N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut

   Plasmid

   #169298

   Purpose

   Constitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Arid3a-3'UTR-Mut (Arid3a Mouse)
   

   Use

   Lentiviral

   Mutation

   Mutations in miR-125b-2 and let-7c binding sites

   Promoter

   EFS

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut

   Plasmid

   #169296

   Purpose

   Constitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   ARID3A-3'UTR-Mut (ARID3A Human)
   

   Use

   Lentiviral

   Mutation

   Mutations in miR-125b-2 and let-7c binding sites

   Promoter

   EFS

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-KIF5AR280H-HA-IRES-Puro

   Plasmid

   #166953

   Purpose

   Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A-R280H (Kif5a Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   KIF5A-R280H

   Promoter

   eF1a

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

   Plasmid

   #156433

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   SORORIN, AID (Cdca5 Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSynap.iAChSnFR-Venus-NULL

   Plasmid

   #137953

   Purpose

   Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Insert

   iAChSnFR (yellow version, non-binding variant)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   cpSFGFP replaced with cpSFVenus; Y140A binding si…

   Promoter

   hSynapsin

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro

    Plasmid

    #25936

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExp-His-zBasic-RBD

    Plasmid

    #194999

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli

    Depositor

    Marko Hyvönen

    Article

    Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

    Insert

    RBD (S SARS-CoV-2)
    

    Tags

    His8-Zbasic

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-eGFP-FHA

    Plasmid

    #114129

    Purpose

    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 2-160

    Promoter

    TRE promoter, Tet ON

    Available Since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid

    #132393

    Purpose

    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Proline 301 to Leucine (P301L)

    Promoter

    TREtight

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-Flag-ER-Hoxb8-IRES-GFP

    Plasmid

    #222292

    Purpose

    Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

    Depositor

    David Sykes, Mark Kamps

    Article

    Wang et al Nat Methods. 2006 Apr;3(4):287-93.

    Insert

    ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    The estrogen receptor hormone binding domain cont…

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid

    #132389

    Purpose

    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAd-M3cherry

    Plasmid

    #61041

    Purpose

    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.

    Depositor

    Qiao Zhou

    Article

    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.

    Inserts

    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    

    Use

    Adenoviral

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156429

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    DELTA1-265

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid

    #179905

    Purpose

    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

    Insert

    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-TEAD4-YBD

    Plasmid

    #166450

    Purpose

    Expresses fusion of mCherry and TEAD4-YAP binding domain

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, TEAD4-YBD(217-434) (TEAD4, YAP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156434

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    All zinc fingers mutated

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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