Addgene

We narrowed to 1,057 results for: 1145

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0.22 hA33 luciferase

Plasmid

#68157

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -222 relative to ATG

Promoter

human GPA33 promoter

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pSC-mTDG-K341R-IntE2

Plasmid

#52269

Purpose

Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Use

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K341R, SUMO acceptor site mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Expression

Mutation

deleted R4-R23 deleted exons 71-78

Promoter

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB kd K106R

Plasmid

#108493

Purpose

expression of EGFP-AuroraB kinase dead (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_KLF4

Plasmid

#26815

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Sep 29. ():.

Insert

5'UTR-KLF4-3'UTR (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Starts from second ATG

Promoter

Available since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pSIN4-CMV-K2M

Plasmid

#21164

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Klf4, cMyc (MYC, KLF4 Human)

Use

Lentiviral; Reprogramming

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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ITGA6-bio-His

Plasmid

#53352

Purpose

Expresses full-length Integrin alpha-6 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITGA6 (ITGA6 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-Aurora B WT

Plasmid

#184042

Purpose

Expresses H2B-GFP fused to Aurora B WT

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Use

Tags

EGFP and H2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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His-FLAG-Tev_ACTA1 (alpha-actin)

Plasmid

#188452

Purpose

Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

ACTA1 (ACTA1 Human)

Use

Tags

6xHis-FLAG

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TET-mKLF4

Plasmid

#20906

Purpose

piggyBac vector with tet inducible mKLF4

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

KLF4 (Klf4 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-FL

Plasmid

#34597

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pWPXL-Klf4

Plasmid

#36981

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 6, 2012

Availability

Academic Institutions and Nonprofits only
lenti-HA-mBec1

Plasmid

#99505

Purpose

3rd generation lentiviral expression of wild-type Becn1

Depositor

Congcong He

Article

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEIGW-human KLF4

Plasmid

#100131

Purpose

Human KLF4 lentiviral expression vector

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

Kruppel like factor 4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

E1F

Available since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBpuro - HA KLF4 FL

Plasmid

#34589

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Klf4

Plasmid

#172870

Purpose

Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Klf4 (Klf4 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCX4-KLF4

Plasmid

#36118

Depositor

Steven Dowdy

Article

Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

Insert

kruppel-like factor 4 (KLF4 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO Myc ArB WT RNAiR

Plasmid

#59806

Purpose

Allows the integration of Myc ArB in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

RNAi resistant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Klf4

Plasmid

#136613

Purpose

Dox-inducible lentiviral vector expressing mouse Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Klf4-pA-pgk-hph

Plasmid

#74906

Purpose

Mammalian expression of mKlf4

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Klf4 (Klf4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154G/H250Y

Plasmid

#108492

Purpose

expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154G/H250Y

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-Flag-AuroraB KD (K106R)

Plasmid

#108488

Purpose

expression of Flag-AuroraB kinase death (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pShuttle-EGFP-CLASP2 340-1362 9xS/A

Plasmid

#24373

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) 9xS/A (CLASP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K5S

Plasmid

#34598

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Fragment 1781-2086 of KLF4

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pLJD-KLF4-ER

Plasmid

#34596

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

Myc epitope tag and estrogen receptor fusion

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pAdEasy-EGFP-CLASP2 340-1084 9xS/A

Plasmid

#24525

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) 9xS/A (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5 TO FRT Myc ArB K106R

Plasmid

#59807

Purpose

Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K106R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSN67

Plasmid

#100124

Purpose

expresses putative KLF4 DBD with a 6xHis tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Insert corresponds to the DBD of KLF4

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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