161,570 results

1. pCMV-PE6a

   Plasmid

   #207851

   Purpose

   Mammalian expression of PE6a prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6a
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROSA26-ERT2CreERT2

   Plasmid

   #149436

   Purpose

   ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Insert

   ERT2-Cre-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-mCherry-p2A-Chrimson-TS

   Plasmid

   #158702

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   TRE-mcherry-p2A-Chrimson-TS
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. NeonGreen

   Plasmid

   #197924

   Purpose

   Monomer expression vector

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

   Insert

   NeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH124-ssr

   Plasmid

   #198025

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Ap/Cb resistance cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ApR promoter

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV Blast DEST (706-1)

   Plasmid

   #17451

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-DIO-NaChBac-dTomato

   Plasmid

   #236252

   Purpose

   Cre dependent NaChBac-TdTomato expression

   Depositor

   Janelia Viral Tools

   Article

   Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

   Insert

   NaChBac-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-EGFP-Rac1(Q61L)

   Plasmid

   #13720

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   EGFP-Rac1(Q61L) (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIMAY*

   Plasmid

   #121441

   Purpose

   Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

   Depositor

   Angelika Grundling

   Article

   Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

   Type

   Empty backbone

   Use

   Escherichia coli/staphylococcus aureus shuttle ve…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA HA HP1 alpha

    Plasmid

    #24078

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 VDAC1-TC

    Plasmid

    #211733

    Purpose

    Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Use

    Tags

    Tetracysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-R-Rac1

    Plasmid

    #180576

    Purpose

    ddRFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGal4AD-CIB1

    Plasmid

    #28245

    Purpose

    Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    Gal4AD (CIB1, GAL4 Budding Yeast)
    

    Use

    Tags

    CIB1

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVS1-P-CAG-GFPluc2

    Plasmid

    #80493

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    GFPluc2
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-HA-GFP

    Plasmid

    #22612

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. mRuby2-N1

    Plasmid

    #54614

    Purpose

    Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEGFP-C1-AR V7

    Plasmid

    #86856

    Purpose

    Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

    Depositor

    Marco Marcelli, Michael Mancini

    Article

    Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Alternative splice variant 7 (alteration/deletion…

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT375

    Plasmid

    #125616

    Purpose

    Expresses evoAPOBEC1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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