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shYAP1 # 1

Plasmid

#42540

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5 TrkA

Plasmid

#15002

Depositor

Raymond Birge

Article

Yano et al J Neurosci Res. 2000 Feb 1. 59(3):356-64

Insert

TrkA (NTRK1 Human)

Expression

Mammalian

Mutation

V289L

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-3xrGBD

Plasmid

#176091

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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RED

Plasmid

#139835

Purpose

Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

dCas9 protein contains two mutations: D10A and H8…

Promoter

tet-inducible CMV

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCE-GFP

Plasmid

#41858

Purpose

Non-integrating (episomal) expression of EGFP

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti CMVTRE3G eGFP Puro (w819-1)

Plasmid

#27570

Depositor

Eric Campeau

Insert

Enhance Green Fluorescent Protein

Use

Lentiviral

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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NylCK-TS_pET28a

Plasmid

#215423

Purpose

Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK-TS

Expression

Bacterial

Mutation

S111G/A137L

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEVOL-NBK-1

Plasmid

#207639

Purpose

A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)

Expression

Bacterial

Mutation

The evolved synthetase NBK-1 contains mutations Y…

Promoter

araBAD

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-EV-IRES-GFP

Plasmid

#187640

Purpose

Empty Vector control modified to express GFP

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

EV

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Immunostealth

Plasmid

#239446

Purpose

Expresses murine NIS (mNIS) in mammalian cells for non-immunogenic in vivo imaging using SPECT or PET

Depositor

Scott Lyons

Article

Merrill et al Cell Stress. 2023 Aug 14;7(8):59-68. doi

Insert

sodium iodide symporter (Slc5a5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-tmpknot-epegRNA-puro backbone

Plasmid

#214089

Purpose

Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1c (SREBF1 Human)

Tags

2XFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1

Plasmid

#78834

Purpose

To overexpress RIPK1 in Mammalian Cell

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGY18

Plasmid

#221708

Purpose

Empty Cas9 vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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#2.4 pre-G-SynapShot-TagBFP

Plasmid

#214721

Purpose

Expresses green #2.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 pre-G-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CAPN1

Plasmid

#60941

Purpose

Expresses calpain1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain1 (CAPN1 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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prhaBAD-pA-Hia5

Plasmid

#222303

Purpose

Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

pA-Hia5

Tags

6His and protein A

Expression

Bacterial

Mutation

codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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