We narrowed to 18,127 results for: Mut

1. pX458-HypaCas9

   Plasmid

   #190604

   Purpose

   PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

   Plasmid

   #50461

   Purpose

   Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-mCherry (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA and mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TALED_Left-ND1-AD-E1347A

   Plasmid

   #183894

   Purpose

   Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJL1 sortase

   Plasmid

   #106288

   Purpose

   Expresses sortase in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   sortase A pentamutant
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

   Plasmid

   #50460

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

   Plasmid

   #50463

   Purpose

   Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCaggs-mGold

   Plasmid

   #157996

   Purpose

   Expression of mGold in mammalian cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   mGold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mGold is mVenus with L46F;T63S mutations

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p426MET25_sfpHluorin (MRV55)

   Plasmid

   #115697

   Purpose

   superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   sfpHluorin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. APEX2 - Tubulin in pEGFP

   Plasmid

   #66171

   Purpose

   APEX2 fused to the N-terminus of alpha tubulin

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 - alpha tubulin
   

   Use

   Tags

   Flag epitope tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   APEX2 contains 4 mutations relative to wt soybean…

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRAM29

    Plasmid

    #234315

    Purpose

    G264A Cat-RNA U1367, Catalytically dead ribozyme control

    Depositor

    James Chappell

    Article

    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

    Insert

    Splicing-deficient mutant of ribozyme
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    P. CymRC

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CF-Inp(D281A)

    Plasmid

    #20156

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-Inp54p (D281A) (INP54 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Stop codon at position 331. D281A mutation.

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD.Sigma32.I54N

    Plasmid

    #59982

    Purpose

    Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

    Depositor

    Jeffery Kelly

    Article

    Zhang et al ACS Chem Biol. 2014 Jul 22.

    Insert

    Sigma32 I54N
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I54N

    Promoter

    Available Since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GAG-Pan

    Plasmid

    #99640

    Purpose

    EGFP-mCherry Met71GAG reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Met71 mutated to GAG (Glu)

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GAA-Pan

    Plasmid

    #99639

    Purpose

    EGFP-mCherry Met71GAA reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Met71 mutated to GAA (Glu)

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Casp3 C163A-myc

    Plasmid

    #11814

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 catalytic mutant (CASP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C163A

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQAT10

    Plasmid

    #223382

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TALED_Right-ND1-AD

    Plasmid

    #183900

    Purpose

    Expresses TALED_Right-ND1-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TALED_Left-ND1-E1347A

    Plasmid

    #183895

    Purpose

    Expresses TALED_Left-ND1-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p70a-T7RNAP

    Plasmid

    #105683

    Purpose

    Expresses T7 polymerase in cell-free TXTL.

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid

    #159904

    Purpose

    Mutagenesis of Kcnn3

    Depositor

    Larry Zweifel

    Insert

    Kcnn3 (Kcnn3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQAT11

    Plasmid

    #223383

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQAT08

    Plasmid

    #223380

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQFT-mNGsacB

    Plasmid

    #203676

    Purpose

    pQFT with mNeonGreen and sacB under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Has synonymous (silent) mutations to remove BsrGI…

    Promoter

    PQJ

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid

    #20298

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Promoter

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMX hCD4

    Plasmid

    #14614

    Depositor

    Dan Littman

    Article

    Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

    Insert

    CD4 (CD4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid

    #20297

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid

    #159916

    Purpose

    Mutagenesis of Penk

    Depositor

    Larry Zweifel

    Insert

    Penk (Penk Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS-lpp0.2-K64A

    Plasmid

    #201789

    Purpose

    mutant of PadR; transcriptional regulator can respond to p-coumaric acid

    Depositor

    Yajun Yan

    Article

    Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

    Insert

    PadR (padR Bacillus subtilis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K64A

    Promoter

    Available Since

    July 12, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pSAM_Bt

    Plasmid

    #112497

    Purpose

    Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

    Depositor

    Jeffrey Gordon, Andrew Goodman

    Article

    Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

    Inserts

    INSeq-adapted mariner transposon
    Himar1C9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. His6TEV-UbG76C

    Plasmid

    #75299

    Purpose

    Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    human Ubiquitin mutant G76 to C
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Glycine 76 to Cystine

    Promoter

    T7

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMUH_unc84_2XGFP

    Plasmid

    #46023

    Depositor

    Sean Eddy

    Article

    Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

    Insert

    UNC-84 (unc-84 )
    

    Use

    10xuas-phi31-expression vector

    Tags

    2XGFP

    Expression

    Mutation

    Two amino acid mutations (F279L and L500P) compar…

    Promoter

    10XUAS

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDisplay-Rncp-iGluSnFR1

    Plasmid

    #107336

    Purpose

    Red fluorescent glutamate sensor with ncp topology. Membrane-displayed

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    Rncp-iGluSnFR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid

    #109121

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ

    Depositor

    Allen Institute for Cell Science

    Insert

    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pBTK562 (pDS-GFP)

    Plasmid

    #183129

    Purpose

    Control plasmid that expresses off-target dsRNA

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al Science. 2020 Jan 31;367(6477):573-576.

    Insert

    GFPmut3 (no RBS)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FnR8-10

    Plasmid

    #182569

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AICSDP-58: NUP153-mEGFP

    Plasmid

    #114407

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153

    Depositor

    Allen Institute for Cell Science

    Insert

    NUP153 Homology Arms with mEGFP-linker (NUP153 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    Sept. 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge