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pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL_U2AF1_S34F_mCherry

Plasmid

#84019

Purpose

expression of mutant U2AF1 in mammalian cells

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Mutation

S34F

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSimpleII-NLS-NmCas9-HA-NLS(s)

Plasmid

#47867

Purpose

This plasmid encode NmCas9 with two NLS and a HA tag

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC3

Plasmid

#183299

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23A

Plasmid

#91138

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-KRAB

Plasmid

#92361

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-KRAB

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT1

Plasmid

#183282

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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gh123

Plasmid

#106790

Purpose

expression of gRNA targeting ST8SIA3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA3 (ST8SIA3 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-pX330-Cas9-Blast

Plasmid

#159741

Purpose

Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Promoter

T7

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-KRaION1

Plasmid

#177799

Purpose

Bacterial expression of green fluorescent potassium indicator KRaION1

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION1

Expression

Bacterial

Promoter

araB

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHeEf1a-mEGFP-NLS

Plasmid

#205012

Purpose

The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

Depositor

Kazuharu Arakawa

Article

Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

Insert

mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris

Use

Tardigrade expression

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Expression

Mammalian

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ERBB2

Plasmid

#183287

Purpose

All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ERBB2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_502_sgCD4

Plasmid

#158704

Purpose

CD4 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD4 (CD4 Human)

Use

Lentiviral

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_B2520

Plasmid

#91077

Purpose

Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_EPHA2

Plasmid

#183286

Purpose

All-in-One CRISPRko system with a guide RNA that targets EPHA2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

EPHA2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCF287_EFS-TiCas9-P2A-Puro

Plasmid

#211646

Purpose

EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Tamoxifen-inducible SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFETCh_RAD21

Plasmid

#64056

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RAD21 Homology Arms (RAD21 Human)

Tags

3X Flag P2A Neo

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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