59,541 results

1. SRC_HUMAN_D0

   Plasmid

   #79700

   Purpose

   This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SRC (SRC Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCRII_TOPO_hNEAT1

   Plasmid

   #61518

   Purpose

   human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

   Depositor

   Archa Fox

   Article

   Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

   Insert

   NEAT1 (NEAT1 Human)
   

   Use

   General cloning vector

   Mutation

   *see comment below

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

   Plasmid

   #103870

   Purpose

   High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP NLS Beta-Actin S14C

   Plasmid

   #60614

   Purpose

   Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   S14C

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnCS_SEPT6_SEPT7-TEV-Strep

   Plasmid

   #174499

   Purpose

   bacterial co-expression of human SEPT6 and of human SEPT7

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN6 (SEPTIN6 Human)
   SEPTIN7 (SEPTIN7 Human)
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-neo-PP1-Gamma

   Plasmid

   #171270

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1

   Depositor

   Steven Balk

   Insert

   3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSSRB070_pAC8-hsCRBN

   Plasmid

   #218789

   Purpose

   Insect cell expression vector for FLAG-TEV-Spy-hsCRBN

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   Protein cereblon (CRBN Human)
   

   Tags

   Flag-TEV-Spy

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-puro-shNT

   Plasmid

   #109012

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting shRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6 promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX ALIX-GFP WT

   Plasmid

   #138582

   Purpose

   Expresses fluorescent ALIX in mammalian cells

   Depositor

   Wesley Sundquist

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Lentiviral

   Tags

   GFP and h30

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.22-PGK1-HRE::dLUC

    Plasmid

    #128095

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Promoter

    inserted PGK1 hypoxia response elements (HRE) (x3)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAH1 (lenti hLPL-FLAG)

    Plasmid

    #102448

    Purpose

    FLAG-tagged human LPL in lentiviral vector

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    Lipoprotein lipase (LPL Human)
    

    Use

    Lentiviral

    Tags

    6x His and FLAG

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. F2R-DuET

    Plasmid

    #213234

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    F2R (F2R Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTOR_1 shRNA

    Plasmid

    #1855

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    mTOR (MTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJC49

    Plasmid

    #133060

    Purpose

    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mScarletI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0078 RESCUE

    Plasmid

    #130661

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd (RESCUE)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

    Plasmid

    #232879

    Purpose

    Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    5-Hydroxytryptamine Receptor 6 (HTR6 Human)
    

    Use

    Lentiviral

    Tags

    RGECO1.1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. dPspCas13b-mutFTO

    Plasmid

    #223697

    Purpose

    Expressing fusion protein of dPspCas13b and inactive mutant of human FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    dPspCas13b-GGS-mutFTO (FTO Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HBM-Luc

    Plasmid

    #35155

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 2.5 kb of the c-myc minimal promoter

    Promoter

    c-myc

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetrosuper Myc shRNA

    Plasmid

    #15662

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    Myc shRNA (MYC Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Mutation

    see publication for details

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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