We narrowed to 13,645 results for: sequence
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Plasmid#213400PurposePlant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursorDepositorInsertArabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
ExpressionPlantPromoter2x35SAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-mNFAT1(2-144)
Plasmid#11108PurposeExpresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
TagsGAL4-DBDExpressionMammalianMutationContains NFAT1 amino acids 2-144; L78P and P140Q …Available SinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_007
Plasmid#123760PurposeEncodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK systemDepositorInsertPTS1
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR(T/A)
Plasmid#118473PurposeNegative-control mutant for blueCKAR biosensor.DepositorInsertblueCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-CeNLuc
Plasmid#135948PurposeExpresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertCeNLuc LumiFluor
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TmCA-P(11)4
Plasmid#185783PurposeEncodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTmCA-P(11)4
TagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 MOV10 K530A
Plasmid#51713Purposeplasmid contains the coding sequence of MOV10 K530ADepositorAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-H4
Plasmid#202178PurposeLevel 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertH4 terminator
UseSynthetic BiologyMutationNoneAvailable SinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-fcpA
Plasmid#202164PurposeLevel 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertfcpA terminator
UseSynthetic BiologyMutationNoneAvailable SinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/CGA-mEGFP-IRES-mCherry
Plasmid#49233PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21-MRPP1_native+
Plasmid#67865Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form)DepositorInsertMRPP1 (TRMT10C Human)
ExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_ss-SBP-EGFP
Plasmid#172358PurposeReporter to monitoring a streptavidin-binding peptide traffickingDepositorInsertER importing signal sequence
UseLentiviralTagsSBP-EGFPExpressionMammalianPromoterCMVAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTagBFP2-WPRE
Plasmid#193332PurposeExpresses mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
ExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/CGA-mEGFP-IRES-mCherry
Plasmid#49224PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Sox17(2A-phiC31o)
Plasmid#140762PurposeTargeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.DepositorInsertphiC31o
UseMouse TargetingPromoterEndogenous Sox17Available SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS1-mEGFP-NLS
Plasmid#205013PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mCherry-NLS
Plasmid#205009PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AID-BFP-loxP_myo2_neoR
Plasmid#194054PurposeDual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genomeDepositorAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTL-Bag2T-gRNA2
Plasmid#196253PurposePlasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAsDepositorInsertCRISPR-Cas9 guide RNA scaffold and multiple cloning site
ExpressionBacterialPromoterJ23119 promoterAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sox1(2A-Cre)
Plasmid#140760PurposeTargeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.DepositorInsertCre
UseMouse TargetingPromoterEndogenous Sox1Available SinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIhh-5A9-LUC
Plasmid#46318DepositorInsert5 copies of A9 sequence from Ihh promoter
TagsluciferaseExpressionMammalianPromotermouse osteocalcin gene 2 promoterAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
D44_Ld_180090
Plasmid#135858PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertalpha glucosidase II subunit, putative
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK206
Plasmid#110588PurposeBTK Type 3 coding sequence for golden gate assemblyDepositorInsertNanoluc
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLI_BFP_C-tag TNF
Plasmid#171187Purposeinducible expression of C-tag TNFDepositorInsertC-tag TNF
UseLentiviralTagsC-tag (internal)ExpressionMammalianMutationADAM cleavage site of TNF (AA73-76) was exchanged…Promotertight TRE promoterAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/UAA-mEGFP-IRES-mCherry
Plasmid#49225PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shCUX1-5328
Plasmid#100815PurposeLentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099DepositorInsertTGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
UseLentiviralExpressionMammalianAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
COG4 shRNA
Plasmid#163861PurposeshRNA targeting the coding sequences of COG4DepositorInsertcomponent of oligomeric golgi complex 4 (COG4 Human)
ExpressionMammalianAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_E323K_TEV
Plasmid#47699PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323KDepositorInsertMaltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
TagsMBPExpressionBacterialMutationchanged glutamic acid 323 to lysinePromoterPtacAvailable SinceSept. 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits