We narrowed to 11,691 results for: NSI

1. pYSD153

   Plasmid

   #212929

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_PACSIN2-1/1

   Plasmid

   #91330

   Purpose

   Protein expression and purification of human SH3 domain construct PACSIN2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PACSIN2-1/1 (PACSIN2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ptdEos-CLC

   Plasmid

   #38009

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Inserts

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   Eos FP, tandem dimer
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_SH3PXD2A-5/5

   Plasmid

   #91464

   Purpose

   Protein expression and purification of human SH3 domain construct SH3PXD2A-5/5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3PXD2A-5/5 (SH3PXD2A Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_SH3PXD2A-1/5

   Plasmid

   #91351

   Purpose

   Protein expression and purification of human SH3 domain construct SH3PXD2A-1/5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3PXD2A-1/5 (SH3PXD2A Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_SH3PXD2A-3/5

   Plasmid

   #91352

   Purpose

   Protein expression and purification of human SH3 domain construct SH3PXD2A-3/5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3PXD2A-3/5 (SH3PXD2A Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE80l B-CN-B

   Plasmid

   #196477

   Purpose

   Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   B-CN-B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG.Myc-SARS2_3CL

   Plasmid

   #200120

   Purpose

   Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-26 (GB1001)

    Plasmid

    #68208

    Purpose

    Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtU6-26 promoter
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-ExRai-AKAR-Kras

    Plasmid

    #118408

    Purpose

    Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-Kras
    

    Tags

    C-terminal targeting sequence from Kras

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHsp18.2 (GB0153)

    Plasmid

    #68171

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK503

    Plasmid

    #155380

    Purpose

    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    May 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. BAS288 C terminal mKO2 natR

    Plasmid

    #74083

    Purpose

    S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    mKO2
    

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pHH0103_SNX9-1/1

    Plasmid

    #91513

    Purpose

    Protein expression and purification of human SH3 domain construct SNX9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SNX9-1/1 (SNX9 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM2-rPMCA2a

    Plasmid

    #47762

    Purpose

    mammalian expression of rat PMCA2a

    Depositor

    Emanuel Strehler, John Penniston

    Article

    Elwess et al J Biol Chem. 1997 Jul 18;272(29):17981-6

    Insert

    PMCA2a (Atp2b2 Rat)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-RAB-AKARev(T/A)

    Plasmid

    #118479

    Purpose

    Negative-control mutant for RAB-AKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. NELFE-mOrange pHis-//

    Plasmid

    #53303

    Purpose

    Expresses His-tagged NELF-E-mOrange fusion protein.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Insert

    NELF-E protein (Nelf-E Fly)
    

    Tags

    His and mOrange

    Expression

    Bacterial

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-CRY2-mCherry

    Plasmid

    #178525

    Purpose

    Expresses CRY2-mCherry in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGB Tnos:NptII:Pnos (GB0226)

    Plasmid

    #68212

    Purpose

    Transcriptional unit for kanamycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    NptII
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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