164,223 results

1. HMGA2 shRNA

   Plasmid

   #25408

   Depositor

   Sean Morrison

   Article

   Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

   Insert

   high mobility group AT-Hook 2 (Hmga2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221-Hyg

   Plasmid

   #48994

   Purpose

   Gateway adapted transfer of Hygromycin resistance gene

   Depositor

   Rick Tarleton

   Article

   Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

   Insert

   hygromycin B phosphotransferase
   

   Use

   Gateway attb1 & attb2

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. AAV hSYN DIO mGluR1-myc-FLAG

   Plasmid

   #166227

   Purpose

   AAV expressing cre-dependent mGluR1-myc-FLAG

   Depositor

   Jason Aoto

   Article

   Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

   Insert

   metabotropic glutamate receptor 1 (Grm1 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CW3SL-EGFP

   Plasmid

   #61463

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHygCG2

   Plasmid

   #200073

   Purpose

   hygR selection mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

   Plasmid

   #207123

   Purpose

   Lentiviral vector to express human METTL3

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   hMETTL3 (METTL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLexM-SLC7A11

   Plasmid

   #197993

   Purpose

   Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-synapsin-HaloCaMP1b-EGFP

   Plasmid

   #138328

   Purpose

   chemigenetic calcium indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Has Service

   AAV1 and AAV9

   Insert

   HaloCaMP1b
   

   Use

   AAV

   Tags

   EGFP, His-tag, and Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp-DNAseI

   Plasmid

   #182409

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-DNase I (DNASE1 Bovine)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tol2 8xSTAR-sTomato-NLS. PGK-puro

    Plasmid

    #136261

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOT_2 - lenti-EFS-tNGFR-2A-puro

    Plasmid

    #181971

    Purpose

    Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    tNGFR (NGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    truncation: aa 11-276

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-DRD2-cpHalo∆-mEGFP

    Plasmid

    #194679

    Purpose

    CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    DRD2_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid

    #46912

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-VP64-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    3xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. RSV-Flag-Brd4

    Plasmid

    #86616

    Purpose

    Flag tagged mouse Brd4 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd4 (Brd4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-CD28

    Plasmid

    #223596

    Purpose

    Lentiviral expression of human CD28 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB_TRE3G_scFV-sfGFP-Prdm9CD

    Plasmid

    #235577

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. WT GLUT1-GFP

    Plasmid

    #200093

    Purpose

    WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    GLUT1 (SLC2A1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HBT-CPK32-Flag

    Plasmid

    #107432

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    CPK32 (CPK32 Mustard Weed)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCM351

    Plasmid

    #46013

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    na

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-N-Puro-3xHA

    Plasmid

    #207696

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. YFP-Rac1

    Plasmid

    #11391

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2+-eGFP

    Plasmid

    #221710

    Purpose

    Expression of eGFP in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    eGFP
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

    Plasmid

    #121448

    Purpose

    To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

    Depositor

    Brian Chen

    Article

    Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

    Insert

    RFPnols-PQR-ZeocinR (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

    Plasmid

    #163031

    Purpose

    FLiCRE TF with fast kinetics for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    Nrxn3b-MKII-f-hLOV1-TEVcs-tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171617

    Purpose

    AAV Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-hERalpha

    Plasmid

    #101141

    Purpose

    Mammalian expression of full-length human estrogen receptor-alpha

    Depositor

    Elizabeth Wilson

    Article

    Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

    Insert

    estrogen receptor-alpha (ESR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Kohinoor-Vimentin/pcDNA3

    Plasmid

    #67772

    Purpose

    Vimentin-targeted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor-Vimentin
    

    Use

    Tags

    Vimentin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

28. TFORF1800

    Plasmid

    #143546

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    USF2 (USF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF2884

    Plasmid

    #143654

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FIGLA (FIGLA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge