176,041 results

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Publication

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D770-N771 insNPG

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
AAVS1 pegRNA2 with trimmed attP

Plasmid

#222347

Purpose

pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA2

Use

CRISPR

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag

Plasmid

#208051

Purpose

Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag

Depositor

Quanfu Ma

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pOG44_FLP_recombinase (pEHA1338)

Plasmid

#209087

Purpose

FLP recombinase for FRT-mediated integration

Depositor

Vikram Khurana

Publication

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Insert

FLP recombinase

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAGGS SARS-CoV-2 BA.4/5 Spike

Plasmid

#186031

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

Depositor

Marceline Côté

Publication

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.4/5 Spike (S )

Expression

Mammalian

Mutation

BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Publication

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Tags

His tag

Expression

Bacterial

Promoter

T7 Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

Plasmid

#166055

Purpose

Expresses the full length TurboID on the surface of astrocytes

Depositor

Scott Soderling

Publication

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-N-Myc_WT USP7

Plasmid

#131242

Purpose

Mammalian expression of N-terminally Myc-tagged USP7

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Publication

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3M14nes

Plasmid

#155367

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Use

CRISPR

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223 SARS-CoV-2 NSP14

Plasmid

#141267

Purpose

Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Publication

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpyTag003-MBP

Plasmid

#133450

Purpose

Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGL3basic luciferase and renilla_polyA

Plasmid

#128046

Purpose

pGL3 basic luciferase reporter with co-expression of renilla luciferase

Depositor

Oskar Laur

Type

Empty backbone

Expression

Mammalian

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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STING-V1

Plasmid

#124262

Purpose

C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Tags

Venus1

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Tom20-V5-FKBP-AP_pLX304

Plasmid

#120914

Purpose

Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

Tom20-V5-FKBP-AP

Use

Lentiviral

Expression

Mammalian

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

Plasmid

#107943

Purpose

Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain

Tags

3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

Expression

Mammalian

Mutation

E174R, S542R, K548R and D908A

Promoter

CAG

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Expression

Mammalian

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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