We narrowed to 30,619 results for: ica

1. AAV.hSyn.GLPLight-ctr

   Plasmid

   #187468

   Purpose

   Expresses GLPLight-ctr in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight-ctr
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. OSCA1.2

   Plasmid

   #136593

   Purpose

   Expresses the mechanosensitive channel OSCA1.2 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   OSCA1.2 (AT4G22120 Mustard Weed)
   

   Use

   Tags

   pIRES2-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-MRGBP

   Plasmid

   #15325

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

   Insert

   MRG-binding protein (MRGBP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pFLAG/HA-DGCR8

   Plasmid

   #10921

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

   Insert

   DGCR8 (DGCR8 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-His-SCov2-nsp8

   Plasmid

   #154758

   Purpose

   Expression of SARS-CoV-2 nsp8

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME-lamp1

   Plasmid

   #109559

   Purpose

   Multisite gateway vector for 5' tagging with lamp1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   lamp1 (lamp1b Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.cytGAP

   Plasmid

   #78734

   Purpose

   Expression of Ca2+ sensor GAP in the cytosol

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   cytGAP
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hMTP

   Plasmid

   #124281

   Purpose

   Expresses human MTP in mammalian cells.

   Depositor

   Mahmood Hussain

   Article

   Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

   Insert

   MTTP (MTTP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET20b-NLuc-MBP-6xHis

   Plasmid

   #135931

   Purpose

   Expresses NanoLuciferase in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SpyCatcher002-oTet

    Plasmid

    #124663

    Purpose

    Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTet
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHiUGE HA-epitope donor ORF2

    Plasmid

    #200373

    Purpose

    Tagging endogenously expressed proteins with HA epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    HA epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMBP-SpyCatcher

    Plasmid

    #79053

    Purpose

    Expresses maltose binding protein (MBP) SpyCatcher fusion

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    MBP-SpyCatcher
    

    Use

    Tags

    6xHis and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Derived from pDEST14-SpyCatcher (Plasmid #35044)…

    Promoter

    T7

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-hTimeless (pc4729)

    Plasmid

    #216861

    Purpose

    Mammalian expression vector encoding GFP tagged human Timeless

    Depositor

    Cristina Cardoso

    Article

    Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDAS12125_PEAR-GFP

    Plasmid

    #177178

    Purpose

    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hsyn-flex-Positron-ST

    Plasmid

    #129267

    Purpose

    Cre-dependant expression of soma-localized Positron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron-ST
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    hsyn

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-TetOn-NLS-mCherry

    Plasmid

    #193762

    Purpose

    Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    NLS-mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    SV40 NLS fused to mCherry

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-mCherry-Ki67

    Plasmid

    #183750

    Purpose

    Expresses EGFP-mCherry-Ki67

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFW2000

    Plasmid

    #224211

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SpyCatcher002-oTri

    Plasmid

    #124662

    Purpose

    Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTri
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClCry4 PHR

    Plasmid

    #160552

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 HR
    

    Use

    Tags

    Hexa his

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. lenti GFAABC1D-dCas9-KRAB-MeCP2

    Plasmid

    #194701

    Purpose

    Expresses dCas9-KRAB-MeCP2 in Astrocytes

    Depositor

    Timothy Jarome

    Article

    Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

    Insert

    GFAABC1D promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    None

    Promoter

    GFAABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-FKBP-SAC1

    Plasmid

    #108127

    Purpose

    Recruitable SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NLS-SuperNova

    Plasmid

    #131858

    Purpose

    Expresses SuperNova fused to nuclear localization signal (NLS)

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Use

    Tags

    Myc tag and c-myc nuclear localization signal (NL…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. human p53-(311-393)

    Plasmid

    #24869

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 311-393.

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pAAV-CMV-CaM-V5-sTb(N)

    Plasmid

    #219779

    Purpose

    sTb(N) half of CaST for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CaM-V5-sTb(N)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CMV-CD4-sTb(C)-M13-GFP

    Plasmid

    #219780

    Purpose

    sTb(C) half CaST for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTb(C)-M13-GFP
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSB700 lenti gRNA mcherry

    Plasmid

    #167910

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p6610 MSCV-IP N-HAonly TIMP1

    Plasmid

    #35009

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    TIMP1 (TIMP1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

30. p5E-smyhc1

    Plasmid

    #126748

    Purpose

    Zebrafish slow myosin heavy chain promoter. Clone 667

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    smyhc1 (smyhc1 Zebrafish)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p-G4PID-HA

    Plasmid

    #209639

    Purpose

    Expression G4PID-HA in mammalian cells

    Depositor

    Xiang Zhou

    Article

    Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

    Insert

    G4PID (DHX36 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAWP78-PVCpnf_pvc13-truncated

    Plasmid

    #198286

    Purpose

    Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-truncated
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RIP Cre-ER (DM#265)

    Plasmid

    #15029

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    Rat insulin promoter (RIP) (Ins2 Rat)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Cre-ER (TM)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. groSL-GFP

    Plasmid

    #109389

    Purpose

    GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pgroSL-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p210 pCMV-CREM

    Plasmid

    #8395

    Purpose

    Cre with chimeric/β-globin intron within the Cre coding sequence

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLH-sgRNA-Sirius-4X(MS2-PP7)

    Plasmid

    #121941

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-4X(MS2-PP7)
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1(-) FLIPQTV3.0 100micro

    Plasmid

    #63730

    Purpose

    FLIPQTV3.0 glutamine sensor with D157N mutation

    Depositor

    Sakiko Okumoto

    Article

    Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

    Insert

    FLIPQTV3.0 sensor, 100microM affinity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D157N in glutamine binding domain

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

    Plasmid

    #75203

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p{CFD4-EYFP-3xP3::DsRed}

    Plasmid

    #86863

    Purpose

    Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Nano-lantern(cAMP-1.6)/pRSETB

    Plasmid

    #53591

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only