163,445 results

1. pX330S-2

   Plasmid

   #58778

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOMM20-mTurquoise-Rac1-G12V-deltaCaaX

   Plasmid

   #176124

   Purpose

   Constitutively active, mitochondrial localized Rac1

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-MAPTau-C-10

   Plasmid

   #55077

   Purpose

   Localization: Microtubules, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   MAPTau (MAPT Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-GFP

   Plasmid

   #160697

   Purpose

   Expresses GFP in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hUbc13

   Plasmid

   #51131

   Purpose

   human ubc13, E2 for synthesis k63 linked ubiquitin chain

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

   Insert

   ubc13 (UBE2N Human)
   

   Use

   Tags

   His6 and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. phL1A-pcDNA3

   Plasmid

   #12307

   Depositor

   Vance Lemmon

   Article

   Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMD2.G-K47Q/R354Q

   Plasmid

   #207323

   Purpose

   VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q/R354Q

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-NLRC5

   Plasmid

   #37521

   Depositor

   Thomas Kufer

   Article

   Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

   Insert

   NLRC5 (NLRC5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T-E2-25K

   Plasmid

   #63572

   Purpose

   Expression of human Ubiquitin conjugating enzyme E2-25K

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBE2-25K (UBA7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Isoform 1 (200 resiudes)

   Promoter

   tac

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEF6-Lck-TurboID

    Plasmid

    #159433

    Purpose

    expresses Lck-TurboID in mammalian cells

    Depositor

    Arthur Salomon

    Article

    Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

    Inserts

    Lck (LCK Human)
    TurboID (BirA mutant)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    EF-1a

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pScarlet-N1

    Plasmid

    #128060

    Purpose

    Mammalian expression vector for C terminal mScarlet-I fusions

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRham-CALM1

    Plasmid

    #182500

    Purpose

    Bacterial expression of the Homo sapiens calmodulin, CALM1

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    CALM1 (CALM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-Hb9-GFP

    Plasmid

    #37080

    Purpose

    3rd generation lentiviral transfer plasmid for Hb9 GFP

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hb9

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. LifeAct-HaloTag

    Plasmid

    #176105

    Purpose

    F-actin marker

    Depositor

    Dorus Gadella

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PiggyBac PlayBack

    Plasmid

    #203312

    Purpose

    To all for PiggyBac gene insertion into Genome

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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