We narrowed to 1,483 results for: MBP

1. pMAL c2x Tral N

   Plasmid

   #113009

   Purpose

   For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   N terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 1-355

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL c2x Tral C

   Plasmid

   #113008

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 356-652

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Plasmid

   #101207

   Purpose

   Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A and Q695A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Plasmid

   #101214

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/K855A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C and K855A

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hSRAP.HMG6.wt.Dlnk.a13-236

   Plasmid

   #49814

   Purpose

   expression vector for MBP-SRAP(13-236) fusion

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator protein (SRA1 Human)
   

   Use

   Tags

   E. coli Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL-XPG

   Plasmid

   #39215

   Depositor

   Aziz Sancar

   Article

   Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

   Insert

   XPG (ERCC5 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Note that XPG starts at amino acid 5

   Promoter

   Ptac

   Available since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. prhaBAD-anti-mouse-Nb-Hia5

   Plasmid

   #239626

   Purpose

   Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

   Depositor

   Aaron Straight

   Article

   Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

   Insert

   Hia5
   

   Use

   Tags

   6HIS, MBP, TEV, and anti-Mouse Nanobody

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   rhaBAD

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF-rM1PYK

    Plasmid

    #220232

    Purpose

    Inducible expression of rabbit muscle pyruvate kinase isoform 1

    Depositor

    Liskin Swint-Kruse, Aron Fenton

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Rabbit muscle pyruvate kinase (PKM )
    

    Use

    Tags

    6xHis-MBP-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-LRRK2 WD40

    Plasmid

    #139211

    Purpose

    Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

    Depositor

    Hao Wu

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    N-His and MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid

    #101217

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDest-636

    Plasmid

    #159574

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    His6-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid

    #101230

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid

    #101211

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid

    #101204

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid

    #101231

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDS170-enNTS1

    Plasmid

    #199152

    Purpose

    JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

    Depositor

    Daniel Scott

    Article

    Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

    Insert

    enNTS1 (Ntsr1 Rat)
    

    Use

    Tags

    10xHis tag, Avi tag, MBP, and muGFP

    Expression

    Bacterial

    Mutation

    V208M, C273S, C391S and C393S (over NTS1-B5)

    Promoter

    Lac/T5

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMTTH+mEGFP-LANA-H1.4CTD

    Plasmid

    #157802

    Purpose

    Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA-H1.4CTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid

    #101208

    Purpose

    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A and Q695A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

    Plasmid

    #111052

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AB (325-557, Δ 368-523)

    Promoter

    T7

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHTSHP-Smt-IIShhN

    Plasmid

    #121135

    Purpose

    Expresses N-terminal SUMO tagged II-ShhN in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, MBP, and Smt tag

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-195, with two extra i…

    Promoter

    tac

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid

    #101229

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMAL-c5e-Rubicon RH N821K, T911R, K916R

    Plasmid

    #216707

    Purpose

    Bacterial protein expression of Rubicon RH domain mutant

    Depositor

    James Hurley

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    N821K, T911R, K916R

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hzHDAC10

    Plasmid

    #225542

    Purpose

    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

    Insert

    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Mutated A24E and D94A

    Promoter

    T7

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-FLAG-B-eSpCas9

    Plasmid

    #126772

    Purpose

    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMAL c2x Tral C 15A

    Plasmid

    #113007

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PNG phosphorylation sites mutated to A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMAL c2x Tral FL-15D

    Plasmid

    #113010

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to D

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid

    #101210

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid

    #101203

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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38. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid

    #101212

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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39. pMAL-PIAL2M-wt

    Plasmid

    #67909

    Purpose

    Inducible expression of PIAL2 in E.coli

    Depositor

    Andreas Bachmair

    Article

    Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

    Insert

    PIAL2 (AT5G41580 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    E281-A496

    Promoter

    P-lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

40. Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

    Plasmid

    #111072

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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41. Kv7.4 BD (522-645, C643A)/pET28HMT

    Plasmid

    #111073

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 BD (522-645, C643A)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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42. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

    Plasmid

    #111267

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.5 AB (361-545, Δ395-511)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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43. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. TH1204-pOCC120-FUS_opt(NheI)

    Plasmid

    #221892

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    MBP-PreScission and mGFP-PreScission-6xHis

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. ASC-CASP1 Octamer

    Plasmid

    #164032

    Purpose

    Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CASP1, ASC (CASP1, PYCARD Human)
    

    Use

    Tags

    His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    W169G (ASC), G20K (CASP1)

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET28a Cdk2ap1CAN

    Plasmid

    #178032

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    Promoter

    T7LacI

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pJ4M/TDP-43 S48E

    Plasmid

    #104481

    Purpose

    bacterial expression of TDP-43 S48E

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    S48E

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET-hMICU1EF

    Plasmid

    #199180

    Purpose

    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

    Depositor

    Ming-Feng Tsai

    Article

    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    MICU1 (MICU1 Human)
    

    Use

    Tags

    His6 and MBP, Thrombin site, TEV site

    Expression

    Mutation

    D421A, E432K, F433W, F453W

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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