We narrowed to 17,435 results for: STO

1. pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

   Plasmid

   #32139

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   B3RT> STOP STOP B3RT>
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

   Plasmid

   #32145

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   loxP> STOP STOP loxP>
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

   Plasmid

   #215002

   Purpose

   Shuttling vector for an ERphagy reporter

   Depositor

   Wade Harper

   Insert

   KDEL
   

   Use

   Gateway

   Tags

   HaloTag(7)-mGFP

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]

   Plasmid

   #71809

   Purpose

   Generating transgenic flies for FLP-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p255 pPr-CREM/Ins/CMV-STOP-luc

   Plasmid

   #8408

   Purpose

   Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   CREM/insulators/lox/luciferase
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   modified Cre

   Promoter

   Probasin, CMV

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

6. aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

   Plasmid

   #101298

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-698 of alpha-Catenin

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215014

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

   Plasmid

   #159874

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP

   Plasmid

   #32143

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   RSRT> STOP STOP RSRT>
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP

    Plasmid

    #32147

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    rox> STOP STOP rox>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP

    Plasmid

    #32135

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    B2RT> STOP STOP B2RT>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

    Plasmid

    #162958

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    triple FLAG epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]

    Plasmid

    #71810

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

    Plasmid

    #162967

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Dual Strep2 purification tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid

    #195624

    Purpose

    pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

    Depositor

    Tonia Rex

    Insert

    dCasphi
    

    Use

    AAV

    Tags

    C-Myc (NLS), HA tag, and ZIM3

    Expression

    Mammalian

    Mutation

    P749A and P753A

    Promoter

    EF1a (Mlu1/EcoR1)

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

    Plasmid

    #196887

    Purpose

    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid

    #215011

    Purpose

    Express a Mitophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    pSu9 (Presequence of Fo-ATPase subunit 9)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

    Plasmid

    #207135

    Purpose

    DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

    Plasmid

    #207136

    Purpose

    DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204729

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204727

    Purpose

    Frt-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

    Plasmid

    #196884

    Purpose

    Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-Lyn-GFP-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

    Plasmid

    #191176

    Purpose

    Mammalian expression of EK0209 with a second stop codon.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    CMV-TO:mCherry:s70587:EGFP (double-stop)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    additional stop codon

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

    Plasmid

    #191147

    Purpose

    Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    Bdfn.t+54
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

    Plasmid

    #162936

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

    Plasmid

    #162937

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. C514-E11: attB5r-RU5u-His6-nostop-attB1r

    Plasmid

    #162953

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 purification tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

    Plasmid

    #162935

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Maltose-binding protein fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. C514-E09: attB5r-RU5u-FLAG-nostop-attB1r

    Plasmid

    #162951

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    FLAG epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. C514-E59: attB5r-RU5u-2xHA-nostop-attB1r

    Plasmid

    #162966

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Dual HA epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. C514-E42: attB5r-RU5u-Strep2-nostop-attB1r

    Plasmid

    #162961

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Strep2 purification tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. C514-E26: attB5r-RU5u-myc-nostop-attB1r

    Plasmid

    #162960

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Myc epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

    Plasmid

    #162932

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion with AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMiR-CIP2A-stop-mut-miR-375-mutA-E

    Plasmid

    #53714

    Purpose

    Luciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutations

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on the stop codon of firefly luciferase …

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

38. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

    Plasmid

    #24334

    Depositor

    Didier Stainier

    Article

    Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

    Insert

    H2B
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    mCherry driven by an HSP70 promoterCerulean drive…

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

    Plasmid

    #106171

    Purpose

    cre mediated red to green shift

    Depositor

    Ewa Snaar-Jagalska

    Article

    Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

    Inserts

    dsRED-express2
    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217515

    Purpose

    Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

    Plasmid

    #52889

    Purpose

    "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

    Depositor

    Iswar Hariharan

    Article

    Bosch et al Development. 2015 Feb 1;142(3):597-606.

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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43. MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

    Plasmid

    #128607

    Purpose

    Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    36xMashTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

    Plasmid

    #63169

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-FLAG"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

    Plasmid

    #85041

    Purpose

    This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    loxP-stop-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA

    Plasmid

    #175438

    Purpose

    dual plasmid labelling cells of different neuronal population with different fluorophores

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    rox-STOP-rox-tRFP-pA
    

    Use

    Cre/Lox; Dre/rox

    Tags

    ZsGreen on backbone

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

    Plasmid

    #159859

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217513

    Purpose

    Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. MSCV-Stop-NPM-ALK-MSNA-IRES-EGFP (MSNAIE)

    Plasmid

    #154470

    Purpose

    cDNA of NPM-ALK in the MSE backbone

    Depositor

    Cornelius Miething

    Article

    Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

    Insert

    NPM-ALK
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

    Plasmid

    #52419

    Purpose

    Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    ERAS (ERAS Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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